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Literature summary for 3.1.3.18 extracted from
- Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum (2004), J. Biol. Chem., 279, 517-526.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | activates at chloride ion concentration up to 50 mM | Thermoplasma acidophilum |  |
| KCl | below 50 mM | Thermoplasma acidophilum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| X-ray analysis | Thermoplasma acidophilum |
General Stability
| General Stability | Organism |
|---|---|
| stable | Thermoplasma acidophilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits | Thermoplasma acidophilum | |
| Cl- | inhibits at high concentration, above 50 mM, competitive inhibition; total inhibition at chloride ion concentration of 600 mM | Thermoplasma acidophilum | |
| KCl | total inhibition at chloride ion concentration of 600 mM | Thermoplasma acidophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
2-Phosphoglycolate | pH 7.5, 70°C | Spinacia oleracea | |
| 0.037 | - |
Phosphoglycolate | - |
Thermoplasma acidophilum | |
| 0.037 | - |
2-Phosphoglycolate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 0.061 | - |
2-Phosphoglycolate | pH 7.5, 70°C | Homo sapiens | |
| 0.17 | - |
diphosphate | - |
Thermoplasma acidophilum | |
| 0.17 | - |
p-nitrophenyl phosphate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 3.1 | - |
p-nitrophenyl phosphate | - |
Thermoplasma acidophilum | |
| 3.1 | - |
diphosphate | pH 7.5, 70°C | Thermoplasma acidophilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | activates at low concentration, below 50 | Thermoplasma acidophilum | |
| Co2+ | - |
Thermoplasma acidophilum | |
| Co2+ | activates | Thermoplasma acidophilum | |
| Co2+ | activates, KD = 0.036 +/- 0.004 | Thermoplasma acidophilum | |
| KCl | activates at chloride ion concentration up to 50 mM | Thermoplasma acidophilum | |
| Mg2+ | - |
Thermoplasma acidophilum | |
| Mg2+ | activates | Thermoplasma acidophilum | |
| Mg2+ | activates, KD = 0.13 +/- 0.01 | Thermoplasma acidophilum | |
| Mn2+ | - |
Thermoplasma acidophilum | |
| Mn2+ | activates | Thermoplasma acidophilum | |
| Mn2+ | activates, KD = 0.02 +/- 0.004 | Thermoplasma acidophilum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoglycolate + H2O | Thermoplasma acidophilum | - |
glycolate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
| Thermoplasma acidophilum | Q9HLQ2 | - |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood cell | red | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.94 | - |
p-nitrophenyl phosphate | Thermoplasma acidophilum |
| 11.55 | - |
diphosphate | Thermoplasma acidophilum |
| 19.82 | - |
substrate phosphoglycolate | Thermoplasma acidophilum |
| 19.82 | - |
phosphoglycolate | Thermoplasma acidophilum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 5% glycerol, 0.5 M NaCl, no loss in activity after several months | Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Phosphoglycolate + H2O | - |
Homo sapiens | Glycolate + phosphate | - |
? | |
| 2-Phosphoglycolate + H2O | - |
Spinacia oleracea | Glycolate + phosphate | - |
? | |
| 2-Phosphoglycolate + H2O | - |
Thermoplasma acidophilum | Glycolate + phosphate | - |
? | |
| diphosphate + H2O | - |
Thermoplasma acidophilum | 2 phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Thermoplasma acidophilum | p-nitrophenol + phosphate | - |
? | |
| phosphoglycolate + H2O | - |
Thermoplasma acidophilum | glycolate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | gel filtration | Thermoplasma acidophilum |
| homodimer | gel filtration | Thermoplasma acidophilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | 0.51 | 2-Phosphoglycolate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 0.58 | - |
p-nitrophenyl phosphate | - |
Thermoplasma acidophilum | |
| 0.58 | - |
diphosphate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 4.8 | - |
diphosphate | - |
Thermoplasma acidophilum | |
| 4.8 | - |
p-nitrophenyl phosphate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 6.08 | - |
diphosphate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 8.26 | - |
Phosphoglycolate | - |
Thermoplasma acidophilum | |
| 8.26 | - |
2-Phosphoglycolate | pH 7.5, 70°C | Thermoplasma acidophilum | |
| 19 | - |
2-Phosphoglycolate | pH 7.5, 70°C | Homo sapiens | |
| 292 | - |
2-Phosphoglycolate | pH 7.5, 70°C | Spinacia oleracea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Thermoplasma acidophilum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
Ca2+ | - |
Thermoplasma acidophilum | |
| 18.04 | - |
KCl | - |
Thermoplasma acidophilum | |
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