Literature summary for 3.1.3.17 extracted from

Zhang, Z.; Bai, G.; Deans-Zirattu, S.; Browner, M.F.; Lee, E.Y.C.
Expression of the catalytic subunit of phosphorylase phosphatase (protein phosphatase-1) in Escherichia coli (1992), J. Biol. Chem., 267, 1484-1490.

Search for more literature for 3.1.3.17

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Expression of the enzyme in the pET3a vector results in the expression of the enzyme as an insoluble aggregate. The vector with the trp-lac hybrid promotor the enzyme is expressed as a soluble protein at levels of 3-4% of the soluble Escherichia coli protein	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
microcystin-LR	-	Oryctolagus cuniculus
okadaic acid	-	Oryctolagus cuniculus
protein	modulator protein, inhibitor 2	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	x * 37000, SDS-PAGE	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	skeletal	Oryctolagus cuniculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphorylase a + H2O	-	Oryctolagus cuniculus	phosphorylase b + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Oryctolagus cuniculus
monomer	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)