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Literature summary for 3.1.3.16 extracted from

  • Fortwendel, J.R.; Juvvadi, P.R.; Pinchai, N.; Perfect, B.Z.; Alspaugh, J.A.; Perfect, J.R.; Steinbach, W.J.
    Differential effects of inhibiting chitin and 1,3-{beta}-D-glucan synthesis in ras and calcineurin mutants of Aspergillus fumigatus (2009), Antimicrob. Agents Chemother., 53, 476-482.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant PPIalpha in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E42A site-directed mutagenesis, the mutant shows only slightly reduced sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme Homo sapiens
E42C site-directed mutagenesis, the mutant shows only slightly reduced sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme Homo sapiens
E42T site-directed mutagenesis, the mutant shows only slightly reduced sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme Homo sapiens
V41D site-directed mutagenesis, the mutant shows unaltered sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme Homo sapiens
W43A site-directed mutagenesis, the mutant shows unaltered sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
human Inhibitor-3 possesses a putative protein phosphatase-1 binding motif, 39KKVEW43. A second interaction site, whose deletion leads to loss of inhibitory potency, is identified between residues 65-77 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant PPIalpha from Escherichia coli to near homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphorylated phosphorylase a + H2O
-
Homo sapiens phosphorylase a + phosphate
-
?

Subunits

Subunits Comment Organism
More PP1 possesses a hydrophobic pocket which binds a peptide motif of the type RVXF that is present in most of the known PP1 targeting/regulatory subunits Homo sapiens

Synonyms

Synonyms Comment Organism
PPI
-
Homo sapiens
PPIalpha
-
Homo sapiens
protein phosphatase-1
-
Homo sapiens