Literature summary for 3.1.3.16 extracted from

Dai, J.; Zhang, J.; Sun, Y.; Wu, Q.; Sun, L.; Ji, C.; Gu, S.; Feng, C.; Xie, Y.; Mao, Y.
Characterization of a novel human protein phosphatase 2C family member, PP2Ckappa (2006), Int. J. Mol. Med., 17, 1117-1123.

Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the recombinant Trx-His-tagged enzyme is not affected by 0.001 mM okadaic acid	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent	Homo sapiens
Mn2+	dependent	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	from sequence of cDNA calculated molecular weight is about 46000 kDa	Homo sapiens
66000	-	Trx-His-tagged recombinant enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8N3J5	mitochondrial precursor	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
AD-293 cell	-	Homo sapiens	-
brain	-	Homo sapiens	-
heart	-	Homo sapiens	-
kidney	-	Homo sapiens	-
ovary	-	Homo sapiens	-
pancreas	-	Homo sapiens	-
thymus	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl phosphate + H2O	-	Homo sapiens	p-nitrophenol + phosphate	-	?
phosphothreonine + H2O	-	Homo sapiens	threonine + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PP2Ckappa	isozyme	Homo sapiens
protein phosphatase 2Ckappa	-	Homo sapiens

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Release 2023.1 (January 2023)