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Literature summary for 3.1.3.16 extracted from

  • Terrak, M.; Kerff, F.; Langsetmo, K.; Tao, T.; Dominguez, R.
    Structural basis of protein phosphatase 1 regulation (2004), Nature, 429, 780-784.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of the intein-enzyme fusion protein and the regulatory 34 kDa myosin phosphatase targeting subunit MYPT1 residues 1-299 in Escherichia coli strain BL21(DE3) Gallus gallus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme bound to the regulatory 34 kDa myosin phosphatase targeting subunit MYPT1 residues 1-299, 8 mg/ml protein in 10 mM Tris, pH 7.5, 20 mM NaCl, 4 mM DTT, and 1 mM MnCl2, 20°C, hanging drop vapour diffusion method, mixed with an equal volume of reservoir solution containing 9.2% w/v PEG 5000 monomethylether, 35% v/v glycerol, and 200 mM NH4Cl, X-ray diffraction structure determination and analysis at 2.7 A resolution Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for full activiy Gallus gallus
additional information one of the two metal ions in the metal center of the enzyme might as well be Fe2+ instead of Mn2+ Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus the enzyme is involved in regulation of many biological processes, e.g. glycogen metabolism, cell-cycle progression, and muscle relaxation, regulatory mechanism involving myosin phosphatase targeting subunit MYPT1 in smooth muscle relaxation, overview ?
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?

Organism

Organism UniProt Comment Textmining
Gallus gallus P62207 isozyme delta; PP1 isozyme delta
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Purification (Commentary)

Purification (Comment) Organism
recombinant intein-enzyme fusion protein from Escherichia coli strain BL21(DE3) by chitin affinity chromatography, DTT-induced self-cleavage of the intein tag, followed by ion exchange chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
smooth muscle
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Gallus gallus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in regulation of many biological processes, e.g. glycogen metabolism, cell-cycle progression, and muscle relaxation, regulatory mechanism involving myosin phosphatase targeting subunit MYPT1 in smooth muscle relaxation, overview Gallus gallus ?
-
?
additional information myosin phosphatase targeting subunit MYPT1 increases myosine specificity of PP1, interaction and binding structure, overview Gallus gallus ?
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?

Synonyms

Synonyms Comment Organism
PP1
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Gallus gallus
protein phosphatase 1
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Gallus gallus