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Literature summary for 3.1.3.16 extracted from

  • Li, R.; Potters, M.B.; Shi, L.; Kennelly, P.J.
    The protein phosphatases of Synechocystis sp. strain PCC 6803: open reading frames sll1033 and sll1387 encode enzymes that exhibit both protein-serine and protein-tyrosine phosphatase activity in vitro (2005), J. Bacteriol., 187, 5877-5884.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment) Organism
genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1, DNA and amino acid sequence determination and analysis, expression analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
D415A site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
D415N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
D444N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
D604N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
D608N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate, the activity with phosphotyrosine-RCML is highly increased compared to the wild-type enzyme Synechocystis sp.
D648N site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.
E414Q site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate, the activity with 4-nitropehnyl phosphate is similar to the wild-type enzyme Synechocystis sp.
E618Q site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
 phosphoserine-casein pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
0.00045
-
 phosphoserine-myelin basic protein pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
0.0017
-
 phosphoserine-RCML pH 7.5, 37°, recombinant SynPPP1 Synechocystis sp.
0.0063
-
 phosphoserine-myelin basic protein pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.
0.022
-
 phosphoserine-myelin basic protein pH 7.5, 37°, recombinant SynPPP1 Synechocystis sp.
12.7
-
 4-nitrophenyl phosphate pH 7.5, 37°, recombinant SynPPM3 Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Synechocystis sp.
Mn2+ required Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803, genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged SynPPM3 and SynPPP1 and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Synechocystis sp.

Reaction

Reaction Comment Organism Reaction ID
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate the enzymes of Synechocystis sp. also perform the protein tyrosine phosphatase reaction, EC 3.1.3.48 Synechocystis sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative activities of mutant SynPPM3 compared to wild-type SynPPM3 Synechocystis sp.
0.0001
-
 purified recombinant SynPPM3, substrate phosphotyrosine-casein Synechocystis sp.
0.0007
-
 purified recombinant SynPPM3, substrate phosphotyrosine-RCML Synechocystis sp.
0.0017
-
 purified recombinant SynPPM3, substrate phosphoserine-RCML Synechocystis sp.
0.0017
-
 purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein Synechocystis sp.
0.0029
-
 purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein Synechocystis sp.
0.0034
-
 purified recombinant SynPPP1, substrate phosphoserine-casein Synechocystis sp.
0.0044
-
 purified recombinant SynPPP1, substrate phosphotyrosine-casein Synechocystis sp.
0.0053
-
 purified recombinant SynPPM3, substrate phosphoserine-casein Synechocystis sp.
0.0055
-
 purified recombinant SynPPP1, substrate phosphotyrosine-RCML Synechocystis sp.
0.0121
-
 purified recombinant SynPPP1, substrate phosphoserine-RCML Synechocystis sp.
0.0178
-
 purified recombinant SynPPM3, substrate phosphoserine-myelin basic protein Synechocystis sp.
0.066
-
 purified recombinant SynPPM3, substrate 4-nitrophenyl phosphate Synechocystis sp.
0.084
-
 purified recombinant SynPPP1, substrate 4-nitrophenyl phosphate Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Synechocystis sp. 4-nitrophenol + phosphate
-
 ?
phosphoserine-casein + H2O
-
 Synechocystis sp. casein + phosphate
-
 ?
phosphoserine-myelin basic protein + H2O
-
 Synechocystis sp. myelin basic protein + phosphate
-
 ?
phosphoserine-RCML + H2O i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphoserine Synechocystis sp. RCML + phosphate
-
 ?
phosphotyrosine-casein + H2O
-
 Synechocystis sp. casein + phosphate
-
 ?
phosphotyrosine-myelin basic protein + H2O
-
 Synechocystis sp. myelin basic protein + phosphate
-
 ?
phosphotyrosine-RCML + H2O i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphotyrosine Synechocystis sp. RCML + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.3.48 Synechocystis sp.
protein sewrine/threonine phosphatase
-
 Synechocystis sp.
SynPPM3
-
 Synechocystis sp.
SynPPP1
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Synechocystis sp.