Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of regucalcin-expressing transgenic Sprague-Dawley rats showing reduced protein phosphatase activity in the heart cytosol, overview | Rattus norvegicus |
additional information | construction of transgenic rats expressing the inhibitory protein regucalcin in the heart muscle cell cytosol leading to reduced enzyme activity and abolishing the activating effect of Ca2+, overview | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
regucalcin | has regulatory function in heart cytosol; the protein inhibits the enzyme in presence of EGTA or ethylene glycol and has a regulatory function for the enzyme independently of Ca2+ | Rattus norvegicus | |
trifluoperazin | cocmplete inhibition at 0.001-0.002 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Rattus norvegicus | |
Ca2+ | activates, synergistic with calmodulin, activation is completely inhibited by trifluoroperazine | Rattus norvegicus | |
Calmodulin | activates the enzyme with substratees phospho-L-serine and phospho-L-threonine, but not with phospho-L-tyrosine | Rattus norvegicus | |
Mg2+ | activates | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospho-L-serine + H2O | Rattus norvegicus | - |
L-serine + phosphate | - |
? | |
phospho-L-threonine + H2O | Rattus norvegicus | - |
L-threonine + phosphate | - |
? | |
phospho-L-tyrosine + H2O | Rattus norvegicus | preferred substrate | L-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female and male Wistar rats | - |
Rattus norvegicus | - |
regucalcin-expressing transgenic Sprague-Dawley rats and wild-type male Wistar rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac muscle | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospho-L-serine + H2O | - |
Rattus norvegicus | L-serine + phosphate | - |
? | |
phospho-L-threonine + H2O | - |
Rattus norvegicus | L-threonine + phosphate | - |
? | |
phospho-L-tyrosine + H2O | - |
Rattus norvegicus | L-tyrosine + phosphate | - |
? | |
phospho-L-tyrosine + H2O | preferred substrate | Rattus norvegicus | L-tyrosine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.48, protein-tyrosine phosphatase | Rattus norvegicus |
More | cf. EC 3.1.3.48, protein-tyrosine-phosphatase | Rattus norvegicus |
protein phosphatase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |