Literature summary for 3.1.3.16 extracted from

White, D.J.; Reiter, N.J.; Sikkink, R.A.; Yu, L.; Rusnak, F.
Identification of the high affinity Mn2+ binding site of Bacteriophage l phosphoprotein phosphatase: effects of metal ligand mutations on electron paramagnetic resonance spectra and phosphatase activities (2001), Biochemistry, 40, 8918-8929.

Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Lambdavirus lambda

Protein Variants

Protein Variants	Comment	Organism
H117N	no changes in activity compared to wild-type enzyme	Lambdavirus lambda
H186N	decreased phosphatase activity	Lambdavirus lambda
H22N	decreased phosphatase activity	Lambdavirus lambda
H92N	no changes in activity compared to wild-type enzyme	Lambdavirus lambda
N75H	decreased phosphatase activity	Lambdavirus lambda

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10.3	-	p-nitrophenyl phosphate	pH 7.8, wild-type Prep 2	Lambdavirus lambda
11.4	-	p-nitrophenyl phosphate	pH 7.8, wild-type Prep 1	Lambdavirus lambda
16	-	p-nitrophenyl phosphate	pH 7.8, H92N and H22N	Lambdavirus lambda
17	-	p-nitrophenyl phosphate	pH 7.8, N75H	Lambdavirus lambda
18	-	p-nitrophenyl phosphate	pH 7.8, H117N	Lambdavirus lambda
90	-	p-nitrophenyl phosphate	pH 7.8, H186N	Lambdavirus lambda

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Lambdavirus lambda

Organism

Organism	UniProt	Comment	Textmining
Lambdavirus lambda	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lambdavirus lambda

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl phosphate + H2O	-	Lambdavirus lambda	p-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
LAMBDAPP	-	Lambdavirus lambda

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.1	-	p-nitrophenyl phosphate	pH 7.8, H186N	Lambdavirus lambda
3.1	-	p-nitrophenyl phosphate	pH 7.8, N75H	Lambdavirus lambda
5	-	p-nitrophenyl phosphate	pH 7.8, H22N	Lambdavirus lambda
490	-	p-nitrophenyl phosphate	pH 7.8, H92N	Lambdavirus lambda
500	-	p-nitrophenyl phosphate	pH 7.8, wild-type Prep 2	Lambdavirus lambda
660	-	p-nitrophenyl phosphate	pH 7.8, H117N	Lambdavirus lambda
1100	-	p-nitrophenyl phosphate	pH 7.8, wild-type Prep 1	Lambdavirus lambda

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Release 2023.1 (January 2023)