Literature summary for 3.1.3.16 extracted from

Marley, A.E.; Sullivan, J.E.; Carling, D.; Abbott, W.M.; Smith, G.J.; Taylor, I.W.F.; Carey, F.; Beri, R.K.
Biochemical characterization and deletion analysis of recombinant human protein phosphatase 2C (1996), Biochem. J., 320, 801-806.

No PubMed abstract available

Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
phosphatase PP2Calpha expressed in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
okadaic acid	no inhibition of phosphatase PP2Calpha	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.067	-	FLRT(P)SCG	-	Homo sapiens
0.096	-	FLRTT(P)CG	-	Homo sapiens
0.152	-	RAT(P)VA	-	Homo sapiens
0.71	-	FLRTS(P)CG	-	Homo sapiens
3	-	p-nitrophenyl phosphate	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	phosphatase type PP2Calpha not stimulated	Homo sapiens
Mn2+	activation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PP2Calpha	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
FLRT(P)SCG + H2O	-	Homo sapiens	FLRTSCG + phosphate	-	?
FLRTS(P)CG + H2O	-	Homo sapiens	FLRTSCG + phosphate	-	?
FLRTT(P)CG + H2O	-	Homo sapiens	FLRTTCG + phosphate	-	?
p-nitrophenyl phosphate + H2O	-	Homo sapiens	p-nitrophenol + phosphate	-	?
phosphoproteins + H2O	-	Homo sapiens	proteins + phosphate	-	?
RAT(P)VA + H2O	-	Homo sapiens	RATVA + phosphate	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	8.5	phosphatase PP2Calpha	Homo sapiens

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Release 2023.1 (January 2023)