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Literature summary for 3.1.3.15 extracted from

  • Lee, H.S.; Cho, Y.; Lee, J.H.; Kang, S.G.
    Novel monofunctional histidinol-phosphate phosphatase of the DDDD superfamily of phosphohydrolases (2008), J. Bacteriol., 190, 2629-2632.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
L-histidinol phosphate in the presence of Mn2+, at 80°C and pH 6.5 Thermococcus onnurineus
0.05
-
L-histidinol phosphate in the presence of Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
0.09
-
L-histidinol phosphate in the presence of Mg2+, at 80°C and pH 6.5 Thermococcus onnurineus
0.09
-
L-histidinol phosphate in the presence of Ni2+, at 80°C and pH 6.5 Thermococcus onnurineus
0.59
-
AMP in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
1.45
-
L-phosphoserine in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
2.37
-
D-fructose 6-phosphate in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strong activity in the presence of Co2+ Thermococcus onnurineus
Cu2+ strong activity in the presence of Cu2+ Thermococcus onnurineus
Mg2+ strong activity in the presence of Mg2+ Thermococcus onnurineus
Mn2+ strong activity in the presence of Mn2+ Thermococcus onnurineus
Ni2+ strong activity in the presence of Ni2+ Thermococcus onnurineus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
gel filtration Thermococcus onnurineus

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity column chromatography and Superdex 200 10/300 gel filtration Thermococcus onnurineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O
-
Thermococcus onnurineus ?
-
?
D-fructose 6-phosphate + H2O
-
Thermococcus onnurineus ?
-
?
L-histidinol phosphate + H2O
-
Thermococcus onnurineus L-histidinol + phosphate
-
?
L-phosphoserine + H2O
-
Thermococcus onnurineus L-serine + phosphate
-
?
additional information does not show any phosphatase activity with p-nitrophenyl phosphate Thermococcus onnurineus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 39000, gel filtration Thermococcus onnurineus

Synonyms

Synonyms Comment Organism
histidinol phosphate phosphatase
-
Thermococcus onnurineus
HolPase member of the DDDD phosphohydrolase/phosphotransferase superfamily Thermococcus onnurineus
TON_0887-derived protein
-
Thermococcus onnurineus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
D-fructose 6-phosphate in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
14
-
L-phosphoserine in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
29
-
AMP in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
260
-
L-histidinol phosphate in the presence of 1 mM Co2+, at 80°C and pH 6.5 Thermococcus onnurineus
290
-
L-histidinol phosphate in the presence of 1 mM Ni2+, at 80°C and pH 6.5 Thermococcus onnurineus
320
-
L-histidinol phosphate in the presence of 1 mM Mn2+, at 80°C and pH 6.5 Thermococcus onnurineus
420
-
L-histidinol phosphate in the presence of 1 mM Mg2+, at 80°C and pH 6.5 Thermococcus onnurineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Thermococcus onnurineus