KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-histidinol phosphate | in the presence of Mn2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
0.05 | - |
L-histidinol phosphate | in the presence of Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
0.09 | - |
L-histidinol phosphate | in the presence of Mg2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
0.09 | - |
L-histidinol phosphate | in the presence of Ni2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
0.59 | - |
AMP | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
1.45 | - |
L-phosphoserine | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
2.37 | - |
D-fructose 6-phosphate | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strong activity in the presence of Co2+ | Thermococcus onnurineus | |
Cu2+ | strong activity in the presence of Cu2+ | Thermococcus onnurineus | |
Mg2+ | strong activity in the presence of Mg2+ | Thermococcus onnurineus | |
Mn2+ | strong activity in the presence of Mn2+ | Thermococcus onnurineus | |
Ni2+ | strong activity in the presence of Ni2+ | Thermococcus onnurineus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
gel filtration | Thermococcus onnurineus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity column chromatography and Superdex 200 10/300 gel filtration | Thermococcus onnurineus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | - |
Thermococcus onnurineus | ? | - |
? | |
D-fructose 6-phosphate + H2O | - |
Thermococcus onnurineus | ? | - |
? | |
L-histidinol phosphate + H2O | - |
Thermococcus onnurineus | L-histidinol + phosphate | - |
? | |
L-phosphoserine + H2O | - |
Thermococcus onnurineus | L-serine + phosphate | - |
? | |
additional information | does not show any phosphatase activity with p-nitrophenyl phosphate | Thermococcus onnurineus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, gel filtration | Thermococcus onnurineus |
Synonyms | Comment | Organism |
---|---|---|
histidinol phosphate phosphatase | - |
Thermococcus onnurineus |
HolPase | member of the DDDD phosphohydrolase/phosphotransferase superfamily | Thermococcus onnurineus |
TON_0887-derived protein | - |
Thermococcus onnurineus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
D-fructose 6-phosphate | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
14 | - |
L-phosphoserine | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
29 | - |
AMP | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
260 | - |
L-histidinol phosphate | in the presence of 1 mM Co2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
290 | - |
L-histidinol phosphate | in the presence of 1 mM Ni2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
320 | - |
L-histidinol phosphate | in the presence of 1 mM Mn2+, at 80°C and pH 6.5 | Thermococcus onnurineus | |
420 | - |
L-histidinol phosphate | in the presence of 1 mM Mg2+, at 80°C and pH 6.5 | Thermococcus onnurineus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermococcus onnurineus |