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Literature summary for 3.1.3.12 extracted from

  • Lahiri, S.; Banerjee, S.; Dutta, T.; Sengupta, S.; Dey, S.; Roy, R.; Sengupta, D.; Chattopadhyay, K.; Ghosh, A.K.
    Enzymatic and regulatory attributes of trehalose-6-phosphate phosphatase from Candida utilis and its role during thermal stress (2014), J. Cell. Physiol., 229, 1245-1255.
    View publication on PubMed
Search for more literature for 3.1.3.12

Inhibitors

Inhibitors Comment Organism Structure
CDTA strong inhibition Cyberlindnera jadinii
EDTA strong inhibition Cyberlindnera jadinii
EGTA strong inhibition Cyberlindnera jadinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.82
-
 trehalose 6-phosphate native enzyme, at pH 8.5 and 30°C Cyberlindnera jadinii
0.9
-
 trehalose 6-phosphate recombinant enzyme, at pH 8.5 and 30°C Cyberlindnera jadinii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ has significant positive effect on enzyme activity Cyberlindnera jadinii
Mg2+ required, almost 10fold increase in activity at 2 mM Cyberlindnera jadinii
Mn2+ has significant positive effect on enzyme activity Cyberlindnera jadinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 x * 82000, SDS-PAGE Cyberlindnera jadinii
82108
-
 x * 82108, MALDI-TOF mass spectrometry Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trehalose 6-phosphate + H2O Cyberlindnera jadinii
-
 trehalose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Protein-Pak DEAE column chromatography and Biosuite 125 gel filtration Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01033
-
 cell lysate, at pH 8.5 and 30°C Cyberlindnera jadinii
0.4505
-
 after 43.6fold purification, at pH 8.5 and 30°C Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with sucrose-6-phosphate, fructose-6-phosphate, glucose-6-phosphate, fructose-6-phosphate, mannose-6-phosphate, 4-nitrophenylphosphate, ATP, and ADP Cyberlindnera jadinii ?
-
 ?
trehalose 6-phosphate + H2O
-
 Cyberlindnera jadinii trehalose + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Cyberlindnera jadinii
? x * 82108, MALDI-TOF mass spectrometry Cyberlindnera jadinii

Synonyms

Synonyms Comment Organism
TPP
-
 Cyberlindnera jadinii
trehalose-6-phosphate phosphatase
-
 Cyberlindnera jadinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Cyberlindnera jadinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is most stable up to 30°C. The enzyme is also able to show almost 50% activity at temperatures as high as 60°C Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Cyberlindnera jadinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12 the enzyme retains almost 60% activity in highly alkaline condition (pH 10.0) or moderately acidic condition (pH 6.0). Almost 40% activity still remains in pH as low as 4.0 or as high as 12.0 Cyberlindnera jadinii

Expression

Organism Comment Expression
Cyberlindnera jadinii almost 1.8fold increase in activity is observed when the concentration of S-adenosyl-L-methionine is increased from 0 to 2 mM up