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Literature summary for 3.1.3.12 extracted from

  • Puttikamonkul, S.; Willger, S.; Grahl, N.; Perfect, J.; Movahed, N.; Bothner, B.; Park, S.; Paderu, P.; Perlin, D.; Cramer Jr., R.
    Trehalose 6-phosphate phosphatase is required for cell wall integrity and fungal virulence but not trehalose biosynthesis in the human fungal pathogen Aspergillus fumigatus (2010), Mol. Microbiol., 77, 891-911.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene AFUB_043350, DNA and amino acid sequence determination and analysis Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
additional information generation of a null mutant of orlA by disruption of the coding sequence in the uracil/uridine auxotrophic Aspergillus fumigatus strain CEA17 with the selectable marker pyrG from Aspergillus parasiticus, phenotype and quantitative real-time PCR analysis of cell wall biosynthesis genes in the orlA mutant and wild type, overview Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
gene AFUB_043350
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Aspergillus fumigatus A1163
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gene AFUB_043350
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose 6-phosphate + H2O
-
Aspergillus fumigatus alpha,alpha-trehalose + phosphate
-
?
alpha,alpha-trehalose 6-phosphate + H2O
-
Aspergillus fumigatus A1163 alpha,alpha-trehalose + phosphate
-
?

Synonyms

Synonyms Comment Organism
OrlA
-
Aspergillus fumigatus
T6PP
-
Aspergillus fumigatus
trehalose 6-phosphate phosphatase
-
Aspergillus fumigatus

General Information

General Information Comment Organism
malfunction T6PP mutant OrlA displays severe morphological defects related to asexual reproduction when grown on glucose minimal medium. The production of conidia is highly reduced in the temperature-sensitive mutant. The defects can be rescued partially by growth at below 30°C and fully by addition of osmotic stabilizers, e.g. 1.2 M sorbitol or 1% glycerol, reduction in incubation temperature or increase in glucose levels. The orlA mutant is virtually avirulent in two distinct murine models of invasive pulmonary aspergillosis, mutant hyphae are clearly dysmorphic with shorter length compartments between septa and truncated hyphal tips with knobby, abnormal branching, phenotype, overview Aspergillus fumigatus
metabolism Aspergillus fumigatus contains two trehalose phosphorylases that may be responsible for trehalose production in the absence of OrlA, potential activation of trehalose phosphorylase enzymes by depleted free Pi levels in the orlA mutant, overview Aspergillus fumigatus
physiological function trehalose 6-phosphate phosphatase is required for cell wall integrity and fungal virulence but not trehalose biosynthesis. The trehalose biosynthesis pathway is critical for virulence in human and plant fungal pathogens. Cell wall biosynthesis and trehalose 6-phosphate levels are linked Aspergillus fumigatus