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Literature summary for 3.1.3.12 extracted from
- Cloning and expression of the trehalose-phosphate phosphatase of Mycobacterium tuberculosis: comparison to the enzyme from Mycobacterium smegmatis (2004), Arch. Biochem. Biophys., 426, 250-257.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Rv3372, expression of His-tagged enzyme in Escherichia coli strains DH5alpha and HMS-F | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Diumycin | stimulation at low concentration, inhibition at high concentration | Mycobacterium tuberculosis | |
| Moenomycin | stimulation at low concentration, inhibition at high concentration | Mycobacterium tuberculosis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely dependent on | Mycobacterium tuberculosis | |
| additional information | nearly no activity with Mn2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + H2O | Mycobacterium tuberculosis | - |
trehalose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
gene Rv3372 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + H2O | - |
Mycobacterium tuberculosis | trehalose + phosphate | - |
? | |
| trehalose 6-phosphate + H2O | the enzyme is absolutely specific for trehalose 6-phosphate | Mycobacterium tuberculosis | trehalose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPP | - |
Mycobacterium tuberculosis |
| trehalose-P phosphatase | - |
Mycobacterium tuberculosis |
| trehalose-phosphate phosphatase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycobacterium tuberculosis |
| 60 | - |
complete loss of activity within 1 min, recombinant His-tagged enzyme | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Mycobacterium tuberculosis |
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