Any feedback?
Literature summary for 3.1.3.12 extracted from
- Purification, cloning, expression, and properties of mycobacterial trehalose-phosphate phosphatase (2003), J. Biol. Chem., 278, 2093-2100.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme can play a key role in the biosynthesis of trehalose compounds, such as trehalose mycolates, and may represent an excellent target site for chemotherapy against tuberculosis and other mycobacterial diseases | Mycolicibacterium smegmatis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholate | even 0.025% | Mycolicibacterium smegmatis |  |
| Diumycin | - |
Mycolicibacterium smegmatis | |
| Moenomycin | - |
Mycolicibacterium smegmatis | |
| additional information | not: 10 mM sodium fluoride, 10 mM sodium orthovanadate, Nonident P-40, Triton X-100, octyl glucoside | Mycolicibacterium smegmatis | |
| Sarkosyl | even 0.025% | Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
trehalose 6-phosphate | pH 7.5, 30°C | Mycolicibacterium smegmatis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimal concentration of native 1-2 mM, optimal concentration of recombinant enzyme 3-4 mM | Mycolicibacterium smegmatis | |
| Mn2+ | the native enzyme is less effective than Mg2+, the recombinant enzyme shows equal activity with both Mn2+ and Mg2+ | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mycolicibacterium smegmatis | the enzyme has essentially no activity with any other sugar phosphates | ? | - |
? | |
| trehalose 6-phosphate + H2O | Mycolicibacterium smegmatis | - |
trehalose + phosphate | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | the enzyme is solubilized in presence of 0.2% sarkosyl | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable at least several weeks | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme has essentially no activity with any other sugar phosphates | Mycolicibacterium smegmatis | ? | - |
? | |
| trehalose 6-phosphate + H2O | - |
Mycolicibacterium smegmatis | trehalose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | enzyme is stable to heating or 6 min | Mycolicibacterium smegmatis |
| 70 | - |
enzyme is slowly inactivated | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Mycolicibacterium smegmatis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
