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Literature summary for 3.1.3.12 extracted from
- Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli (2000), Appl. Environ. Microbiol., 66, 2484-2490.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the chimeric gene is expressed in Escherichia coli | Escherichia coli |
| the chimeric gene is expressed in Escherichia coli, a bifunctional fusion enzyme is constructed by fusing genes for trehalose 6-phosphate synthetase and trehalose 6-phosphate phosphatase, this enzyme catalysing sequential reactions has kinetic advantages over a mixture of both enzymes | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
trehalose 6-phosphate | pH 7.5, 30°C | Escherichia coli |  |
| 2.6 | - |
trehalose 6-phosphate | pH 7.5, 30°C, chimeric enzyme | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37400 | - |
SDS-PAGE | Escherichia coli |
| 88300 | - |
chimeric enzyme which contains trehalose 6-phosphate synthetase and trehalose 6-phosphate phosphatase, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + H2O | Escherichia coli | - |
trehalose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | a bifunctional fusion enzyme is constructed by fusing genes for trehalose 6-phosphate synthetase and trehalose 6-phosphate phosphatase, this enzyme catalysing sequential reactions has kinetic advantages over a mixture of both enzymes | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the chimeric gene | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + H2O | - |
Escherichia coli | trehalose + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37400, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | both the chimeric enzyme and the equimolar mixture of trehalose 6-phosphate synthetase/trehalose 6-phosphate phosphatase | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the termal stability of chimeric enzyme and equimolare mixture are quite similar | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.3 | - |
trehalose 6-phosphate | pH 7.5, 30°C | Escherichia coli | |
| 14.6 | - |
trehalose 6-phosphate | pH 7.5, 30°C, chimeric enzyme | Escherichia coli | |
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