Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
K112A | mutations in the AMP-binding site demonstrates an eight to nine-fold decrease in AMP inhibition | Sus scrofa |
L56A | mutant enzyme (interfacial mutant) exhibits an increase in Ki for fructose-2,6-bisphosphate by approximately 5fold | Sus scrofa |
L73A | mutant enzyme (interfacial mutant) exhibits an increase in Ki for fructose-2,6-bisphosphate by approximately 5fold | Sus scrofa |
M177A | mutation reveals an approximate two to three-fold decrease in inhibitory constants (Ki's) for natural inhibitors AMP and fructose-2,6-bisphosphate compared with the wild-type enzyme | Sus scrofa |
M248D | the active site mutant enzyme displays an approximate 7fold increase in Ki for fructose-2,6-bisphosphate. The mutant enzyme displays a four-fold decrease in its apparent Michelis constant, and a six-fold increase in catalytic efficiency | Sus scrofa |
Y113A | mutations in the AMP-binding site demonstrates an eight to nine-fold decrease in AMP inhibition | Sus scrofa |
Y164A | mutation reveals an approximate two to three-fold decrease in inhibitory constants (Ki's) for natural inhibitors AMP and fructose-2,6-bisphosphate compared with the wild-type enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Sus scrofa | |
fructose 2,6-bisphosphate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
D-fructose 1,6-bisphosphate | mutant enzyme M248D, pH 7.5, 37°C | Sus scrofa | |
0.0025 | - |
D-fructose 1,6-bisphosphate | mutant enzyme Y113A, pH 7.5, 37°C | Sus scrofa | |
0.00325 | - |
D-fructose 1,6-bisphosphate | mutant enzyme K112A, pH 7.5, 37°C | Sus scrofa | |
0.0042 | - |
D-fructose 1,6-bisphosphate | mutant enzyme M177A, pH 7.5, 37°C | Sus scrofa | |
0.0048 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, pH 7.5, 37°C | Sus scrofa | |
0.0051 | - |
D-fructose 1,6-bisphosphate | mutant enzyme L73A, pH 7.5, 37°C | Sus scrofa | |
0.0052 | - |
D-fructose 1,6-bisphosphate | mutant enzyme L56A, pH 7.5, 37°C | Sus scrofa | |
0.0053 | - |
D-fructose 1,6-bisphosphate | mutant enzyme Y164A, pH 7.5, 37°C | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Sus scrofa | - |
D-fructose 6-phosphate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P00636 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
FBPase | - |
Sus scrofa |
fructose-1,6-bisphosphatase | - |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
melting point of mutant enzyme Y113A, without ligand | Sus scrofa |
51 | - |
melting point of mutant enzyme K112A, without ligand | Sus scrofa |
54.2 | - |
melting point of mutant enzyme M177A, without ligand | Sus scrofa |
55.4 | - |
melting point of mutant enzyme Y164A, without ligand | Sus scrofa |
55.8 | - |
melting point of mutant enzyme L73A, without ligand | Sus scrofa |
56 | - |
melting point of mutant enzyme L56A, without ligand | Sus scrofa |
56 | - |
melting point of wild-type enzyme, without ligand | Sus scrofa |
62 | - |
melting point of mutant enzyme M248D, without ligand | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.8 | - |
D-fructose 1,6-bisphosphate | mutant enzyme M177A, pH 7.5, 37°C | Sus scrofa | |
13.5 | - |
D-fructose 1,6-bisphosphate | mutant enzyme Y164A, pH 7.5, 37°C | Sus scrofa | |
20.5 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, pH 7.5, 37°C | Sus scrofa | |
22.5 | - |
D-fructose 1,6-bisphosphate | mutant enzyme L73A, pH 7.5, 37°C | Sus scrofa | |
25.6 | - |
D-fructose 1,6-bisphosphate | mutant enzyme L56A, pH 7.5, 37°C | Sus scrofa | |
26.8 | - |
D-fructose 1,6-bisphosphate | mutant enzyme K112A, pH 7.5, 37°C | Sus scrofa | |
27.2 | - |
D-fructose 1,6-bisphosphate | mutant enzyme Y113A, pH 7.5, 37°C | Sus scrofa | |
30.5 | - |
D-fructose 1,6-bisphosphate | mutant enzyme M248D, pH 7.5, 37°C | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
fructose 2,6-bisphosphate | mutant enzyme Y164A, pH 7.5, 37°C | Sus scrofa | |
0.00017 | - |
fructose 2,6-bisphosphate | mutant enzyme M177A, pH 7.5, 37°C | Sus scrofa | |
0.00024 | - |
fructose 2,6-bisphosphate | mutant enzyme K112A, pH 7.5, 37°C | Sus scrofa | |
0.00025 | - |
fructose 2,6-bisphosphate | mutant enzyme Y113A, pH 7.5, 37°C | Sus scrofa | |
0.00025 | - |
fructose 2,6-bisphosphate | wild-type enzyme, pH 7.5, 37°C | Sus scrofa | |
0.00125 | - |
fructose 2,6-bisphosphate | mutant enzyme L56A, pH 7.5, 37°C | Sus scrofa | |
0.00135 | - |
fructose 2,6-bisphosphate | mutant enzyme L73A, pH 7.5, 37°C | Sus scrofa | |
0.0015 | - |
fructose 2,6-bisphosphate | mutant enzyme M248D, pH 7.5, 37°C | Sus scrofa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00075 | - |
mutant enzyme M177A, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.00095 | - |
mutant enzyme Y164A, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.0015 | - |
wild-type enzyme, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.0015 | - |
mutant enzyme K112A, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.0018 | - |
mutant enzyme Y113A, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.0025 | - |
mutant enzyme Y164A, pH 7.5, 37°C | Sus scrofa | AMP | |
0.0035 | - |
mutant enzyme M177A, pH 7.5, 37°C | Sus scrofa | AMP | |
0.00695 | - |
mutant enzyme L73A, pH 7.5, 37°C | Sus scrofa | AMP | |
0.0072 | - |
mutant enzyme M248D, pH 7.5, 37°C | Sus scrofa | AMP | |
0.00725 | - |
mutant enzyme L73A, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.00735 | - |
mutant enzyme L56A, pH 7.5, 37°C | Sus scrofa | AMP | |
0.0075 | - |
wild-type enzyme, pH 7.5, 37°C | Sus scrofa | AMP | |
0.0075 | - |
mutant enzyme L56A, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.0105 | - |
mutant enzyme M248D, pH 7.5, 37°C | Sus scrofa | fructose 2,6-bisphosphate | |
0.0486 | - |
mutant enzyme Y113A, pH 7.5, 37°C | Sus scrofa | AMP | |
0.0685 | - |
mutant enzyme K112A, pH 7.5, 37°C | Sus scrofa | AMP |