Cloned (Comment) | Organism |
---|---|
cloned by rapid amplification of cDNA end technique | Scophthalmus maximus |
gene SMAX5B_013904, enzyme is cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis | Scophthalmus maximus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Scophthalmus maximus | - |
D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Scophthalmus maximus | one of the key enzymes in gluconeogenesis | D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scophthalmus maximus | T1W0T2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low enzyme expression level | Scophthalmus maximus | - |
brain | intermediate activity | Scophthalmus maximus | - |
gill | very low enzyme expression level | Scophthalmus maximus | - |
heart | low activity | Scophthalmus maximus | - |
heart | very low enzyme expression level | Scophthalmus maximus | - |
intestine | low activity | Scophthalmus maximus | - |
intestine | low enzyme expression level | Scophthalmus maximus | - |
kidney | low enzyme expression level | Scophthalmus maximus | - |
kidney | intermediate activity | Scophthalmus maximus | - |
liver | high enzyme expression level | Scophthalmus maximus | - |
liver | the highest expression of glucokinase is found in liver, followed by muscle | Scophthalmus maximus | - |
additional information | expression of FBPase is higher in liver than heart and gill, tissue distribution of FBPase mRNA, overview | Scophthalmus maximus | - |
muscle | dorsal white muscle, moderate enzyme expression level | Scophthalmus maximus | - |
muscle | the highest expression of glucokinase is found in liver, followed by muscle | Scophthalmus maximus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Scophthalmus maximus | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | one of the key enzymes in gluconeogenesis | Scophthalmus maximus | D-fructose 6-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FBPase | - |
Scophthalmus maximus |
fructose-1,6-bisphosphatase | - |
Scophthalmus maximus |
SMAX5B_013904 | - |
Scophthalmus maximus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
annealing temperature | Scophthalmus maximus |
Organism | Comment | Expression |
---|---|---|
Scophthalmus maximus | no significant difference in FBPase mRNA levels between all dietary treatments, overview | additional information |
Scophthalmus maximus | activated by dietary carbohydrates | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the carbohydrate metabolism in the carnivorous fish species. Fructose-1,6-bisphosphatase (FBPase) plays a crucial role in glucose metabolism, together with glucokinase. Feeding with dietary carbohydrates causes no significant differences in FBPase activity and mRNA expression in the glucose as well as dextrin group, while both hepatic glucokinase activity and mRNA expression are highly induced in turbot. The increased hepatic glucokinase activity and gene expression indicates that the first step of glycolysis is activated in turbot by dietary carbohydrates | Scophthalmus maximus |
metabolism | one of the key enzymes in gluconeogenesis | Scophthalmus maximus |
physiological function | fructose-1,6-bisphosphatase (FBPase) plays a crucial role in glucose metabolism, together with glucokinase | Scophthalmus maximus |