Protein Variants | Comment | Organism |
---|---|---|
G20D | kcat for fructose 1,6-bisphosphate is 1.62fold lower than wild-type enzyme, Km-value for fructose 1,6-diphosphate is 1.86fold higherr than wild-type enzyme | Salmonella enterica |
T23I | kcat for fructose 1,6-bisphosphate is 1.2fold lower than wild-type enzyme, Km-value for fructose 1,6-diphosphate is 1.3fold lower than wild-type enzyme | Salmonella enterica |
T91I | kcat for fructose 1,6-bisphosphate is 1.1fold higher than wild-type enzyme, Km-value for fructose 1,6-diphosphate is 1.14fold lower than wild-type enzyme | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminoimidazole carboxamide ribotide | inhibition of fructose-1,6-bisphosphatase by aminoimidazole carboxamide ribotide prevents growth of Salmonella enterica purH mutants on glycerol | Salmonella enterica | |
AMP | - |
Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0085 | - |
D-fructose 1,6-bisphosphate | pH 7.5, mutant enzyme T23I | Salmonella enterica | |
0.0095 | - |
D-fructose 1,6-bisphosphate | pH 7.5, mutant enzyme T19I | Salmonella enterica | |
0.0108 | - |
D-fructose 1,6-bisphosphate | pH 7.5, wild-type enzyme | Salmonella enterica | |
0.0201 | - |
D-fructose 1,6-bisphosphate | pH 7.5, mutant enzyme G20D | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Salmonella enterica | D-fructose 6-phosphate + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
D-fructose 1,6-bisphosphate | pH 7.5, mutant enzyme G20D | Salmonella enterica | |
20.4 | - |
D-fructose 1,6-bisphosphate | pH 7.5, mutant enzyme T23I | Salmonella enterica | |
24.3 | - |
D-fructose 1,6-bisphosphate | pH 7.5, wild-type enzyme | Salmonella enterica | |
26.8 | - |
D-fructose 1,6-bisphosphate | pH 7.5, mutant enzyme T19I | Salmonella enterica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
AMP | wild-type enzyme, competitive dissociation constant Kic | Salmonella enterica | |
0.00414 | - |
AMP | wild-type enzym, uncompetitive dissociation constant Kiu | Salmonella enterica | |
0.0373 | - |
aminoimidazole carboxamide ribotide | wild-type enzyme, competitive dissociation constant Kic | Salmonella enterica | |
0.155 | - |
AMP | mutant enzyme T19I, competitive dissociation constant Kic | Salmonella enterica | |
0.165 | - |
aminoimidazole carboxamide ribotide | wild-type enzym, uncompetitive dissociation constant Kiu | Salmonella enterica | |
0.404 | - |
AMP | mutant enzyme T19I, uncompetitive dissociation constant Kiu | Salmonella enterica | |
0.657 | - |
AMP | mutant enzyme G20S, uncompetitive dissociation constant Kiu | Salmonella enterica | |
1.16 | - |
aminoimidazole carboxamide ribotide | mutant enzyme T23I, competitive dissociation constant Kic | Salmonella enterica | |
1.35 | - |
AMP | mutant enzyme G20S, competitive dissociation constant Kic | Salmonella enterica | |
1.45 | - |
AMP | mutant enzyme T23I, competitive dissociation constant Kic | Salmonella enterica | |
1.7 | - |
aminoimidazole carboxamide ribotide | mutant enzyme T19I, competitive dissociation constant Kic | Salmonella enterica | |
2.16 | - |
aminoimidazole carboxamide ribotide | mutant enzyme G20S, competitive dissociation constant Kic | Salmonella enterica | |
3.8 | - |
aminoimidazole carboxamide ribotide | mutant enzyme T23I, uncompetitive dissociation constant Kiu | Salmonella enterica | |
4.61 | - |
aminoimidazole carboxamide ribotide | mutant enzyme G20S, uncompetitive dissociation constant Kiu | Salmonella enterica | |
6.12 | - |
AMP | mutant enzyme T23I, uncompetitive dissociation constant Kiu | Salmonella enterica | |
6.32 | - |
aminoimidazole carboxamide ribotide | mutant enzyme T19I, uncompetitive dissociation constant Kiu | Salmonella enterica |