Literature summary for 3.1.3.11 extracted from

Zhou, R.; Cheng, L.
Biochemical characterization of cytosolic fructose-1,6-bisphosphatase from apple (Malus domestica) leaves (2004), Plant Cell Physiol., 45, 879-886.

Search for more literature for 3.1.3.11

Activating Compound

Activating Compound	Comment	Organism	Structure
ammonium sulfate	10 mM, 127% of initial activity, 50 mM, no residual activity	Malus domestica
additional information	60 min at 60°C, 85% increase in enzyme activity	Malus domestica

Inhibitors

Inhibitors	Comment	Organism	Structure
ammonium sulfate	10 mM, 127% of initial activity, 50 mM, no residual activity	Malus domestica
AMP	non-competitive. AMP, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate interact in a synergistic way to inhibit the enzyme activity	Malus domestica
Ca2+	0.05 mM, 41% residual activity	Malus domestica
Cu2+	1 mM, no residual activity	Malus domestica
D-fructose 1,6-bisphosphate	competitive, changes substrate saturation curve from hyperbolic to sigmoidal. AMP, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate interact in a synergistic way to inhibit the enzyme activity	Malus domestica
D-fructose 6-phosphate	mixed-type. AMP, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate interact in a synergistic way to inhibit the enzyme activity	Malus domestica
Hg2+	0.1 mM, no residual activity	Malus domestica
Li+	-	Malus domestica
additional information	sorbitol increases the susceptibility of the enzyme to inhibition by high concentrations of D-fructose 1,6-bisphosphate	Malus domestica
Zn2+	-	Malus domestica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0031	-	D-fructose 1,6-bisphosphate	-	Malus domestica

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Malus domestica	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	or Mn2+, required. Km-value for Mg2+ is 0.59 mM	Malus domestica
Mn2+	or Mg2+, required. Km-value for Mn2+ is 0.062 mM. Mn2+ enhances Mg2+ activated enzyme activity	Malus domestica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	4 * 37000, SDS-PAGE	Malus domestica
150000	-	gel filtration	Malus domestica

Organism

Organism	UniProt	Comment	Textmining
Malus domestica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Malus domestica

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Malus domestica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	enzyme is highly specific for D-fructose 1,6-bisphosphate. At 1 mM substrate, activity is only 40% of the activity at 0.06 mM substrate	Malus domestica	D-fructose 6-phosphate + phosphate	-	?
additional information	no substrate: D-glucose 1-phosphate, D-glucose 6-phosphate, sorbitol 6-phosphate, phosphoenolpyruvate, D-fructose 6-phosphate, 3-glycerate phosphate, dihydroxyacetone	Malus domestica	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 37000, SDS-PAGE	Malus domestica

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	purification at 4°C, loss of 80% of the enzyme activity	Malus domestica
60	-	60 min, 85% increase in enzyme activity	Malus domestica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8.8	at 5 mM Mg2+	Malus domestica

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.47	-	D-fructose 6-phosphate	-	Malus domestica

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Release 2023.1 (January 2023)