Literature summary for 3.1.3.11 extracted from

Nelson, S.W.; Honzatko, R.B.; Fromm, H.J.
Origin of cooperativity in the activation of fructose-1,6-bisphosphatase by Mg2+ (2004), J. Biol. Chem., 279, 18481-18487.

Search for more literature for 3.1.3.11

Protein Variants

Protein Variants	Comment	Organism
D118A	mutant tetramer with one wild-type subunit and three mutant subunits. Kinetic parameters similar to wild-type, kcat-value is about one-fourth that of wild-type	Sus scrofa
D121A	mutant tetramer with one wild-type subunit and three mutant subunits. Kinetic parameters similar to wild-type, kcat-value is about one-fourth that of wild-type	Sus scrofa
E97A	mutant tetramer with one wild-type subunit and three mutant subunits. Kinetic parameters similar to wild-type, kcat-value is about one-fourth that of wild-type	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	wild-type, 50% inhibition at 0.0016 mM, mutant E97A, 50% inhibition at 0.0038 mM, mutant D118A, 50% inhibition at 0.0069 mM, mutant D121A, 50% inhibition at 0.0074 mM	Sus scrofa
D-fructose 2,6-bisphosphate	-	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	enzyme shows a mechanism of cooperativity that arises from within a single subunit	Sus scrofa
0.0011	-	D-fructose 1,6-bisphosphate	mutant D121A, E-tagged, pH 7.5	Sus scrofa
0.0013	-	D-fructose 1,6-bisphosphate	mutant D118A, E-tagged, pH 7.5	Sus scrofa
0.0018	-	D-fructose 1,6-bisphosphate	wild-type, pH 7.5	Sus scrofa
0.0018	-	D-fructose 1,6-bisphosphate	mutant E97A, E-tagged, pH 7.5	Sus scrofa
0.0022	-	D-fructose 1,6-bisphosphate	wild-type, E-tagged, pH 7.5	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	P00636	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	-	Sus scrofa	D-fructose 6-phosphate + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.9	-	D-fructose 1,6-bisphosphate	mutant D118A, E-tagged, pH 7.5	Sus scrofa
5.3	-	D-fructose 1,6-bisphosphate	mutant E97A, E-tagged, pH 7.5	Sus scrofa
5.8	-	D-fructose 1,6-bisphosphate	mutant D121A, E-tagged, pH 7.5	Sus scrofa
22	-	D-fructose 1,6-bisphosphate	wild-type, pH 7.5	Sus scrofa
23	-	D-fructose 1,6-bisphosphate	wild-type, E-tagged, pH 7.5	Sus scrofa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00011	-	D-fructose 2,6-bisphosphate	wild-type, E-tagged, pH 7.5	Sus scrofa
0.00012	-	D-fructose 2,6-bisphosphate	wild-type, pH 7.5	Sus scrofa
0.00016	-	D-fructose 2,6-bisphosphate	mutant E97A, E-tagged, pH 7.5	Sus scrofa
0.00017	-	D-fructose 2,6-bisphosphate	mutant D118A, E-tagged, pH 7.5	Sus scrofa
0.00061	-	D-fructose 2,6-bisphosphate	mutant D121A, E-tagged, pH 7.5	Sus scrofa

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Release 2023.1 (January 2023)