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Literature summary for 3.1.3.11 extracted from
- Chloroplast fructose-1,6-bisphosphatase from Oryza differs in salt tolerance property from the Porteresia enzyme and is protected by osmolytes (2001), Plant Sci., 160, 1171-1181.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | non-competitive inhibition of cytosolic enzyme, the chloroplastic enzyme is AMP-insensitive | Oryza coarctata |  |
| AMP | non-competitive inhibition of cytosolic enzyme, the chloroplatic enzyme is AMP-insensitive | Oryza sativa | |
| KCl | in vitro: at higher concentration KCl inhibits the salt-sensitive rice, but the salt-tolerant rice remains unaffected | Oryza sativa | |
| additional information | not: NaCl, KCl, NH4Cl | Oryza coarctata | |
| additional information | not: NH4CL | Oryza sativa | |
| NaCl | in vivo: The presence of 100 mM NaCl in the growth medium only marginally alters the cytosolic and chloroplastic enzyme activities of salt-tolerant varieties whereas both the activities decline appreciably in salt-sensitive varieties tested. in vitro: comparison of inhibition of chloroplastic enzyme in a salt-sensitive and a salt-tolerant enzyme, protection against inhibition in salt-sensitve rice by mannitol, inositol, pinitol, sorbitol, trehalose, sucrose and proline | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 37°C, chloroplastic enzyme | Oryza sativa | |
| 0.076 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 37°C, chloroplastic enzyme | Oryza coarctata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Oryza sativa | 9507 | - |
| chloroplast | - |
Oryza coarctata | 9507 | - |
| cytosol | - |
Oryza sativa | 5829 | - |
| cytosol | - |
Oryza coarctata | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Oryza sativa |
| 43000 | - |
4 * 43000, SDS-PAGE | Oryza coarctata |
| 170000 | - |
chloroplastic enzyme | Oryza sativa |
| 170000 | - |
chloroplastic enzyme | Oryza coarctata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza coarctata | - |
- |
- |
| Oryza sativa | - |
seedlings, salt-sensitive and salt-tolerant varieties | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza coarctata |
| salt sensitive enzyme IR26 | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
| leaf | - |
Oryza coarctata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparison of activities of chloroplastic and cytosolic enzyme in three salt-sensitive and seven salt-tolerant varieties in absence and presence of NaCl | Oryza sativa |
| 1.21 | - |
cytoplasmic enzyme | Oryza coarctata |
| 3.67 | - |
chloroplastic enzyme | Oryza coarctata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Oryza sativa | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Oryza coarctata | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Oryza sativa |
| tetramer | 4 * 43000, SDS-PAGE | Oryza coarctata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Oryza sativa |
| 7 | 7.5 | - |
Oryza coarctata |
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