Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-Fluoro-2,4-dinitrobenzene | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
5,5'-dithiobis-(2-nitrobenzoate) | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
nagarse | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
NEM | up to 90% inhibition at pH 6.5-7.0, slight increase of activity at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
p-mercuribenzoate | up to 90% inhibition at pH 6.5-7.0, slight increase of activity at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
papain | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
Pronase | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Fluoro-2,4-dinitrobenzene | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
5,5'-dithiobis(2-nitrobenzoate) | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
AMP | allosteric | Bos taurus | |
fructose 1,6-diphosphate | at high concentrations | Bos taurus | |
Glutaraldehyde | - |
Bos taurus | |
iodoacetate | - |
Bos taurus | |
nagarse | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
NEM | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
p-mercuribenzoate | up to 90% inhibition at pH 6.5-7.0, slight increase at pH 9.0 when Mg2+ is the metal cofactor. 3fold increase of activity at alkaline pH, less activation at neutral pH when Mn2+ is the metal cofactor | Bos taurus | |
papain | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus | |
Pronase | stimulates 2fold to 3fold at pH 9.0, loss of activity at pH 6.5 | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
fructose 1,6-diphosphate | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | divalent cation required. Mn2+ or Mg2+ | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
equilibrium sedimentation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | Bos taurus | enzyme in gluconeogenic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, pH 7.5-8.0, 10 mM Tris, 1.0 mM EDTA, 3.0 mM MgSO4 or 20 mM beta-glycerophosphate, 1.0 mM fructose 1,6-diphosphate, stable for many months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glycerophosphate + H2O | substrate at pH 9.0, no activity at pH 6.5 | Bos taurus | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | enzyme in gluconeogenic pathway | Bos taurus | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | - |
Bos taurus | D-fructose 6-phosphate + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
fructose 1,6-diphosphate, Mg2+ and Mn2+ protect against thermal inactivation | Bos taurus |
50 | - |
15 min, 50% loss of activity | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Bos taurus |