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Literature summary for 3.1.3.11 extracted from
- Fructose-bisphosphatase from spinach leaf chloroplast and cytoplasm (1982), Methods Enzymol., 90, 371-378.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| fructose 1,6-diphosphate | hysteretic activation of the chloroplastic enzyme | Spinacia oleracea |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | inhibits cytoplasmic enzyme, no inhibition of chloroplastic enzyme | Spinacia oleracea | |
| Ca2+ | chloroplastic enzyme | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| cytoplasm | - |
Spinacia oleracea | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
cytoplasmic enzyme | Spinacia oleracea |
| 160000 | - |
chloroplastic enzyme | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62 | - |
cytoplasmic enzyme | Spinacia oleracea |
| 109 | - |
chloroplastic enzyme | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, almost no activity is lost during storage for 6 months, chloroplastic enzyme | Spinacia oleracea |
| -20°C, stable for 2 weeks, 20% loss of activity after 4 weeks, cytoplasmic enzyme | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Spinacia oleracea | D-fructose 6-phosphate + phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
cytoplasmic enzyme | Spinacia oleracea |
| 8.8 | - |
chloroplastic enzyme | Spinacia oleracea |
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Release 2023.1 (January 2023)
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