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Literature summary for 3.1.3.106 extracted from

  • Shekar, S.; Tumaney, A.W.; Rao, T.J.; Rajasekharan, R.
    Isolation of lysophosphatidic acid phosphatase from developing peanut cotyledons (2002), Plant Physiol., 128, 988-996 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.106

Activating Compound

Activating Compound Comment Organism Structure
octylglucoside 10 mM, 2fold incraese in activity Arachis hypogaea
Triton X-100 10 mM, 2fold incraese in activity Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
CHAPS 2 mM, 50% decrease in activity Arachis hypogaea
deoxycholate 2 mM, 50% decrease in activity Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0286
-
 oleoyl-sn-glycerol 3-phosphate pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea
0.0393
-
 stearoyl-sn-glycerol 3-phosphate pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea
0.0479
-
 palmitoyl-sn-glycerol 3-phosphate pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Arachis hypogaea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 8 mM, 40-50% inhibition Arachis hypogaea
Mn2+ 8 mM, 40-50% inhibition Arachis hypogaea
Zn2+ 8 mM, 40-50% inhibition Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oleoyl-sn-glycerol 3-phosphate + H2O Arachis hypogaea
-
 oleoyl-sn-glycerol + phosphate
-
 ?
palmitoyl-sn-glycerol 3-phosphate + H2O Arachis hypogaea
-
 palmitoyl-sn-glycerol + phosphate
-
 ?
stearoyl-sn-glycerol 3-phosphate + H2O Arachis hypogaea
-
 stearoyl-sn-glycerol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Arachis hypogaea
-
leaf
-
 Arachis hypogaea
-
seed immature seed Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
858
-
 substrate oleoyl-sn-glycerol 3-phosphate, pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is specific to lysophosphatidic acid and does not utilize any other substrate such as glycerol-3-phosphate, phosphatidic acid, or p-nitrophenylphosphate Arachis hypogaea ?
-
 ?
oleoyl-sn-glycerol 3-phosphate + H2O
-
 Arachis hypogaea oleoyl-sn-glycerol + phosphate
-
 ?
palmitoyl-sn-glycerol 3-phosphate + H2O
-
 Arachis hypogaea palmitoyl-sn-glycerol + phosphate
-
 ?
stearoyl-sn-glycerol 3-phosphate + H2O
-
 Arachis hypogaea stearoyl-sn-glycerol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39000, SDS-PAGE Arachis hypogaea

Synonyms

Synonyms Comment Organism
LPA phosphatase
-
 Arachis hypogaea
lysophosphatidic acid phosphatase
-
 Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arachis hypogaea