Literature summary for 3.1.3.106 extracted from

Reddy, V.S.; Singh, A.K.; Rajasekharan, R.
The Saccharomyces cerevisiae PHM8 gene encodes a soluble magnesium-dependent lysophosphatidic acid phosphatase (2008), J. Biol. Chem., 283, 8846-8854 .

Search for more literature for 3.1.3.106

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
D58E	inactive	Saccharomyces cerevisiae
D58N	inactive	Saccharomyces cerevisiae
D60E	33.5% of wild-type activity	Saccharomyces cerevisiae
D60N	inactive	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	complete inhibition	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.034	-	oleoyl-sn-glycerol-3-phosphate	pH 6.5, 30°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Saccharomyces cerevisiae	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	76% of the activity with Mg2+	Saccharomyces cerevisiae
Mg2+	required, maximum activity at 2 mM	Saccharomyces cerevisiae
Mn2+	48% of the activity with Mg2+	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae BY4741	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
oleoyl-sn-glycerol-3-phosphate + H2O	-	Saccharomyces cerevisiae	oleoyl-sn-glycerol + phosphate	-	?
oleoyl-sn-glycerol-3-phosphate + H2O	-	Saccharomyces cerevisiae BY4741	oleoyl-sn-glycerol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Phm8	-	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Saccharomyces cerevisiae

Expression

Organism	Comment	Expression
Saccharomyces cerevisiae	expression is upregulated in response to phosphate-starving conditions	up

General Information

General Information	Comment	Organism
physiological function	a gene deletion mutant shows decreased lysophosphatidic acid-hydrolyzing activity under phosphate-limiting conditions. Overexpression of PHM8 in yeast results in an increase in the lysophosphatidic acid phosphatase activity in vivo	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)