Any feedback?
Literature summary for 3.1.3.105 extracted from
- Enzymatic synthesis and semi-preparative isolation of N-acetylmuramic acid 6-phosphate (2017), Carbohydr. Res., 445, 98-103 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
General Stability
| General Stability | Organism |
|---|---|
| the long-term stability of the enzyme in solution at -80°C is increased by the addition of 20% (v/v) glycerol | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
N-acetyl-D-muramate 6-phosphate | at pH 7.6 and 22°C | Pseudomonas putida |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-muramate 6-phosphate + H2O | Pseudomonas putida | - |
N-acetyl-D-muramate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q88M11 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | neither alpha-1-phosphorylated N-acetyl-D-muramate, N-acetyl-D-glucose, or D-glucose nor glucosamine 6-phosphate or D-glucose 6-phosphate is used as a substrate | Pseudomonas putida | ? | - |
? | |
| N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Pseudomonas putida | N-acetyl-D-glucosamine + phosphate | - |
? | |
| N-acetyl-D-muramate 6-phosphate + H2O | - |
Pseudomonas putida | N-acetyl-D-muramate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25890, calculated from amino acid sequence | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mupP | - |
Pseudomonas putida |
| MurNAc 6P phosphatase | - |
Pseudomonas putida |
| N-acetylmuramic acid 6-phosphate phosphatase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 45 | the recombinant enzyme loses about 10% of its activity at 22°C and 70% at 37°C within 40 min and is completely inactivated within 40 min at 45°C. About 50% of its activity remain at 22°C, and about 85% of its activity remain at 37°C | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.88 | - |
N-acetyl-D-muramate 6-phosphate | at pH 7.6 and 22°C | Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | at pH 4.0 and pH 10.0, the enzyme activity is 40 and 60%, respectively, of the maximum | Pseudomonas putida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.84 | - |
N-acetyl-D-muramate 6-phosphate | at pH 7.6 and 22°C | Pseudomonas putida | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
