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Literature summary for 3.1.3.10 extracted from
- Purification, characterization, and gene cloning of glucose-1-phosphatase from Citrobacter braakii (2009), J. Gen. Appl. Microbiol., 55, 345-350.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
D-glucose 6-phosphate | Vmax: 0.8 micromol/min/mg | Citrobacter braakii |  |
| 1.9 | - |
D-glucose 6-phosphate | Vmax: 2.2 micromol/min/mg | Citrobacter braakii | |
| 5.12 | - |
D-glucose 1-phosphate | Vmax: 1.9 micromol/min/mg | Citrobacter braakii | |
| 9 | - |
D-mannose 6-phosphate | Vmax: 2.6 micromol/min/mg | Citrobacter braakii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43710 | - |
calculated from cDNA | Citrobacter braakii |
| 46000 | - |
SDS-PAGE | Citrobacter braakii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter braakii | D3GGN3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| (NH4)2SO4 precipitation, DEAE-sepharose chromatography, CM-sepharose chromatography and FPLC chromatography | Citrobacter braakii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 6-phosphate + H2O | - |
Citrobacter braakii | D-fructose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Citrobacter braakii | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Citrobacter braakii | D-glucose + phosphate | - |
? | |
| D-mannose 6-phosphate + H2O | - |
Citrobacter braakii | D-mannose + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agpC | - |
Citrobacter braakii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at and optimal temperature | Citrobacter braakii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
enzyme ist stable up to 55°C 30 min | Citrobacter braakii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at and optimal pH | Citrobacter braakii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | - |
Citrobacter braakii |
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