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Literature summary for 3.1.3.1 extracted from
- Purification and characterization of alkaline phosphatase from Thermotoga neapolitana (1997), Enzyme Microb. Technol., 21, 335-340.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.183 | - |
p-nitrophenyl phosphate | - |
Thermotoga neapolitana |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | enhances activity, twice as active than with either Zn2+ or Mn2+ | Thermotoga neapolitana | |
| Mn2+ | enhances activity | Thermotoga neapolitana | |
| Zn2+ | enhances activity | Thermotoga neapolitana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Thermotoga neapolitana |
| 87000 | - |
gel filtration | Thermotoga neapolitana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga neapolitana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga neapolitana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 663.3 | - |
- |
Thermotoga neapolitana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-ADP + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
| 5'-ATP + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Thermotoga neapolitana | D-fructose + phosphate | - |
? | |
| fructose 1,6-diphosphate + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
| glucose 1-phosphate + H2O | - |
Thermotoga neapolitana | glucose + phosphate | - |
? | |
| Glucose 6-phosphate + H2O | - |
Thermotoga neapolitana | Glucose + phosphate | - |
? | |
| glycerophosphate + H2O | - |
Thermotoga neapolitana | glycerol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Thermotoga neapolitana | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000, SDS-PAGE | Thermotoga neapolitana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Thermotoga neapolitana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 95 | 60°C: about 45% of maximal activity, 9°C: about 60% of maximal activity | Thermotoga neapolitana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability is increased 30fold by Co2+ and 23fold by Mg2+ | Thermotoga neapolitana |
| 90 | - |
half-life: 238 min, in presence of Co2+ | Thermotoga neapolitana |
| 100 | - |
40 min, in presence of 40 mM Co2+, 3% loss of activity | Thermotoga neapolitana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.9 | - |
- |
Thermotoga neapolitana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11.5 | pH 8.0: about 40% of maximal activity, pH 11.5: about 45% of maximal activity | Thermotoga neapolitana |
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