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Literature summary for 3.1.3.1 extracted from

  • Yuivar, Y.; Barahona, S.; Alcaino, J.; Cifuentes, V.; Baeza, M.
    Biochemical and thermodynamical characterization of glucose oxidase, invertase, and alkaline phosphatase secreted by antarctic yeasts (2017), Front. Mol. Biosci., 4, 86 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.1

Application

Application Comment Organism
diagnostics biosensors [Candida] sake
diagnostics biosensors Wickerhamomyces anomalus
diagnostics biosensors Goffeauzyma gilvescens
diagnostics biosensors Dioszegia sp.
diagnostics biosensors Mrakia psychrophila
food industry marker for milk pasteurization [Candida] sake
food industry marker for milk pasteurization Wickerhamomyces anomalus
food industry marker for milk pasteurization Goffeauzyma gilvescens
food industry marker for milk pasteurization Dioszegia sp.
food industry marker for milk pasteurization Mrakia psychrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 4-nitrophenyl phosphate at pH 9.0 and 47°C Dioszegia sp.
0.59
-
 4-nitrophenyl phosphate pH 9.0, 47°C Dioszegia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 [Candida] sake
-
-
extracellular
-
 Wickerhamomyces anomalus
-
-
extracellular
-
 Goffeauzyma gilvescens
-
-
extracellular
-
 Dioszegia sp.
-
-
extracellular
-
 Mrakia psychrophila
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Goffeauzyma gilvescens
Mg2+ required Dioszegia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 SDS-PAGE Dioszegia sp.

Organism

Organism UniProt Comment Textmining
Dioszegia sp.
-
-
-
Goffeauzyma gilvescens
-
-
-
Mrakia psychrophila
-
-
-
Wickerhamomyces anomalus A0A1E3P771
-
-
Wickerhamomyces anomalus ATCC 58044 A0A1E3P771
-
-
[Candida] sake
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dioszegia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 [Candida] sake 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Wickerhamomyces anomalus 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Goffeauzyma gilvescens 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Dioszegia sp. 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Mrakia psychrophila 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Wickerhamomyces anomalus ATCC 58044 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Dioszegia sp.

Synonyms

Synonyms Comment Organism
ALP
-
 [Candida] sake
ALP
-
 Wickerhamomyces anomalus
ALP
-
 Goffeauzyma gilvescens
ALP
-
 Dioszegia sp.
ALP
-
 Mrakia psychrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
-
 Goffeauzyma gilvescens
47
-
-
 Dioszegia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 55 4°C: 43% of maximal activity, 10°C: 67% of maximal activity, 30°C: about 88% of maximal activity, 55°C: about 60% of maximal activity Dioszegia sp.
22
-
 after 6 h of incubation the percentage of activity is 87% Dioszegia sp.
37
-
 after 6 h of incubation the percentage of activity is 57% Dioszegia sp.
47
-
 after 6 h of incubation the percentage of activity is 40% Dioszegia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 10 the enzyme keeps 67 and 43% of activity after 1 h at 10°C and 4°C, respectively Dioszegia sp.
4 47 the enzyme retains at least 80% activity it is incubated for 1 h at temperature ranging from 4°C to 47°C Dioszegia sp.
22 47 at 22, 37, and 47°C, the enzyme retains 87, 57, and 40% of activity, respectively, after 6 h of incubation, which declines faster at 57 and 60°C Dioszegia sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1400
-
 4-nitrophenyl phosphate at pH 9.0 and 47°C Dioszegia sp.
1400
-
 4-nitrophenyl phosphate pH 9.0, 47°C Dioszegia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Dioszegia sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2400
-
 4-nitrophenyl phosphate at pH 9.0 and 47°C Dioszegia sp.