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Literature summary for 3.1.3.1 extracted from

  • Aiba, H.; Nishiya, Y.; Ojima, Y.; Azuma, M.
    Over-expression, characterization, and modification of highly active alkaline phosphatase from a Shewanella genus bacterium (2017), Biosci. Biotechnol. Biochem., 81, 1994-2001 .
    View publication on PubMed
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
Maleimide
-
 Shewanella sp. T3-3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C600 cells Shewanella sp. T3-3

Protein Variants

Protein Variants Comment Organism
K161S/K184S the mutant shows much higher residual specific activity after maleimide activation than the wild type enzyme Shewanella sp. T3-3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 4-nitrophenyl phosphate at pH 9.8 and 37°C Shewanella sp. T3-3

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Shewanella sp. T3-3
Zn2+ required Shewanella sp. T3-3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Shewanella sp. T3-3

Organism

Organism UniProt Comment Textmining
Shewanella sp. T3-3 A0A218PHN9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methoxy-4-(3-phosphatephenyl)spiro [1,2-dioxetane-3,2'-adamantane] disodium salt + H2O
-
 Shewanella sp. T3-3 ?
-
 ?
4-nitrophenyl phosphate + H2O
-
 Shewanella sp. T3-3 4-nitrophenol + phosphate
-
 ?
[(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)-methanol 1-(dihydrogenphosphate) disodium salt + H2O
-
 Shewanella sp. T3-3 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 49500, SDS-PAGE Shewanella sp. T3-3
homodimer 2 * 45200, calculated from amino acid sequence Shewanella sp. T3-3

Synonyms

Synonyms Comment Organism
APase
-
 Shewanella sp. T3-3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 65 the enzyme remains stable after 24 h incubation between 20 and 65°C Shewanella sp. T3-3

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7000
-
 4-nitrophenyl phosphate at pH 9.8 and 37°C Shewanella sp. T3-3

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 the enzyme remains stable after 24 h incubation between pH 6.0 and 11.0 Shewanella sp. T3-3