Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | NaCl increases the activity and stability of the enzyme. The effect is pH-dependent in the range of pH 7.0-10.0. The relative increase in activity induced by addition of NaCl is 4fold greater at pH 7.0-8.0 than at pH 9.8. Changing the pH from 8.0 to 10.5 gradually decreases the ionic effect to near no effect at pH 10.5 | Vibrio splendidus | |
Sodium acetate | less effective than NaCl in activating the enzyme | Vibrio splendidus | |
Sodium fluoride | less effective than NaCl in activating the enzyme | Vibrio splendidus | |
sodium glutamate | sodium glutamate promotes enzyme activity at up to 200 mM | Vibrio splendidus | |
Sodium nitrate | less effective than NaCl in activating the enzyme | Vibrio splendidus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Lemo21-(DE3) cells | Vibrio splendidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | product inhibition | Vibrio splendidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
4-nitrophenyl phosphate | at pH 9.0 and 25°C | Vibrio splendidus | |
0.025 | - |
4-nitrophenyl phosphate | at pH 8.0 and 25°C | Vibrio splendidus | |
0.026 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 8.0 and 25°C | Vibrio splendidus | |
0.061 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 9.0 and 25°C | Vibrio splendidus | |
0.484 | - |
4-nitrophenyl phosphate | at pH 10.5 and 25°C | Vibrio splendidus | |
1.48 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 10.5 and 25°C | Vibrio splendidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains 1 Mg2+ ion | Vibrio splendidus | |
Zn2+ | contains 2 Zn2+ ions | Vibrio splendidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio splendidus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Vibrio splendidus | 4-nitrophenol + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme has a half-life of less than 1 min when heated at 30°C with no additional ions in the buffer | Vibrio splendidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.6 | - |
4-nitrophenyl phosphate | at pH 8.0 and 25°C | Vibrio splendidus | |
80 | - |
4-nitrophenyl phosphate | at pH 10.5 and 25°C | Vibrio splendidus | |
99 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 10.5 and 25°C | Vibrio splendidus | |
102 | - |
4-nitrophenyl phosphate | at pH 9.0 and 25°C | Vibrio splendidus | |
145 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 8.0 and 25°C | Vibrio splendidus | |
243 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 9.0 and 25°C | Vibrio splendidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
the pH-activity profile shows two maxima at approximately pH 9.0 and 10.2 | Vibrio splendidus |
10.2 | - |
the pH-activity profile shows two maxima at approximately pH 9.0 and 10.2 | Vibrio splendidus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
phosphate | at pH 9.0 and 25°C | Vibrio splendidus | |
0.022 | - |
phosphate | at pH 8.0 and 25°C | Vibrio splendidus | |
0.095 | - |
phosphate | with 0.5 M NaCl, at pH 8.0 and 25°C | Vibrio splendidus | |
0.164 | - |
phosphate | with 0.5 M NaCl, at pH 9.0 and 25°C | Vibrio splendidus | |
1.46 | - |
phosphate | at pH 10.5 and 25°C | Vibrio splendidus | |
8.64 | - |
phosphate | with 0.5 M NaCl, at pH 10.5 and 25°C | Vibrio splendidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 10.5 and 25°C | Vibrio splendidus | |
170 | - |
4-nitrophenyl phosphate | at pH 10.5 and 25°C | Vibrio splendidus | |
1400 | - |
4-nitrophenyl phosphate | at pH 8.0 and 25°C | Vibrio splendidus | |
4000 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 9.0 and 25°C | Vibrio splendidus | |
5500 | - |
4-nitrophenyl phosphate | at pH 9.0 and 25°C | Vibrio splendidus | |
5600 | - |
4-nitrophenyl phosphate | with 0.5 M NaCl, at pH 8.0 and 25°C | Vibrio splendidus |