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Literature summary for 3.1.3.1 extracted from

  • Hjoerleifsson, J.G.; Asgeirsson, B.
    pH-Dependent binding of chloride to a marine alkaline phosphatase affects the catalysis, active site stability, and dimer equilibrium (2017), Biochemistry, 56, 5075-5089 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl NaCl increases the activity and stability of the enzyme. The effect is pH-dependent in the range of pH 7.0-10.0. The relative increase in activity induced by addition of NaCl is 4fold greater at pH 7.0-8.0 than at pH 9.8. Changing the pH from 8.0 to 10.5 gradually decreases the ionic effect to near no effect at pH 10.5 Vibrio splendidus
Sodium acetate less effective than NaCl in activating the enzyme Vibrio splendidus
Sodium fluoride less effective than NaCl in activating the enzyme Vibrio splendidus
sodium glutamate sodium glutamate promotes enzyme activity at up to 200 mM Vibrio splendidus
Sodium nitrate less effective than NaCl in activating the enzyme Vibrio splendidus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Lemo21-(DE3) cells Vibrio splendidus

Inhibitors

Inhibitors Comment Organism Structure
phosphate product inhibition Vibrio splendidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
4-nitrophenyl phosphate at pH 9.0 and 25°C Vibrio splendidus
0.025
-
4-nitrophenyl phosphate at pH 8.0 and 25°C Vibrio splendidus
0.026
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 8.0 and 25°C Vibrio splendidus
0.061
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 9.0 and 25°C Vibrio splendidus
0.484
-
4-nitrophenyl phosphate at pH 10.5 and 25°C Vibrio splendidus
1.48
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 10.5 and 25°C Vibrio splendidus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains 1 Mg2+ ion Vibrio splendidus
Zn2+ contains 2 Zn2+ ions Vibrio splendidus

Organism

Organism UniProt Comment Textmining
Vibrio splendidus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Vibrio splendidus 4-nitrophenol + phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the enzyme has a half-life of less than 1 min when heated at 30°C with no additional ions in the buffer Vibrio splendidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.6
-
4-nitrophenyl phosphate at pH 8.0 and 25°C Vibrio splendidus
80
-
4-nitrophenyl phosphate at pH 10.5 and 25°C Vibrio splendidus
99
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 10.5 and 25°C Vibrio splendidus
102
-
4-nitrophenyl phosphate at pH 9.0 and 25°C Vibrio splendidus
145
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 8.0 and 25°C Vibrio splendidus
243
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 9.0 and 25°C Vibrio splendidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
the pH-activity profile shows two maxima at approximately pH 9.0 and 10.2 Vibrio splendidus
10.2
-
the pH-activity profile shows two maxima at approximately pH 9.0 and 10.2 Vibrio splendidus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
phosphate at pH 9.0 and 25°C Vibrio splendidus
0.022
-
phosphate at pH 8.0 and 25°C Vibrio splendidus
0.095
-
phosphate with 0.5 M NaCl, at pH 8.0 and 25°C Vibrio splendidus
0.164
-
phosphate with 0.5 M NaCl, at pH 9.0 and 25°C Vibrio splendidus
1.46
-
phosphate at pH 10.5 and 25°C Vibrio splendidus
8.64
-
phosphate with 0.5 M NaCl, at pH 10.5 and 25°C Vibrio splendidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
67
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 10.5 and 25°C Vibrio splendidus
170
-
4-nitrophenyl phosphate at pH 10.5 and 25°C Vibrio splendidus
1400
-
4-nitrophenyl phosphate at pH 8.0 and 25°C Vibrio splendidus
4000
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 9.0 and 25°C Vibrio splendidus
5500
-
4-nitrophenyl phosphate at pH 9.0 and 25°C Vibrio splendidus
5600
-
4-nitrophenyl phosphate with 0.5 M NaCl, at pH 8.0 and 25°C Vibrio splendidus