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Literature summary for 3.1.3.1 extracted from
- Cloning, expression and characterization of a highly active thermostable alkaline phosphatase from Bacillus licheniformis MTCC 1483 in Escherichia coli BL21 (DE3) (2016), Appl. Biochem. Microbiol., 52, 358-365 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 30% residual activity at 5 mM | Bacillus licheniformis |  |
| Fe2+ | 10% residual activity at 5 mM | Bacillus licheniformis | |
| Pb2+ | 30% residual activity at 5 mM | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.96 | - |
4-nitrophenyl phosphate | at pH 10.0 and 50°C | Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 115% activity at 5 mM | Bacillus licheniformis | |
| Co2+ | 108% activity at 5 mM | Bacillus licheniformis | |
| Cu2+ | 112% activity at 2 mM | Bacillus licheniformis | |
| Mg2+ | Mg2+ (5 mM) enhances the enzyme activity by 72% | Bacillus licheniformis | |
| Mn2+ | Mn2+ (2 mM) enhances the enzyme activity by 26% | Bacillus licheniformis | |
| Zn2+ | 118% activity at 5 mM | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | U6A566 | - |
- |
| Bacillus licheniformis MTCC 1483 | U6A566 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Bacillus licheniformis | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Bacillus licheniformis MTCC 1483 | 4-nitrophenol + phosphate | - |
? | |
| 5'-AMP + H2O | - |
Bacillus licheniformis | adenosine + phosphate | - |
? | |
| 5'-AMP + H2O | - |
Bacillus licheniformis MTCC 1483 | adenosine + phosphate | - |
? | |
| alpha-glycerol phosphate + H2O | - |
Bacillus licheniformis | glycerol + phosphate | - |
? | |
| ATP + H2O | - |
Bacillus licheniformis | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Bacillus licheniformis MTCC 1483 | ADP + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Bacillus licheniformis | D-glucose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Bacillus licheniformis MTCC 1483 | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Bacillus licheniformis | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Bacillus licheniformis MTCC 1483 | D-glucose + phosphate | - |
? | |
| linear DNA fragment + H2O | the enzyme has a dephosphorylation efficiency of 92.9% to dephosphorylate linear DNA fragments of plasmid pUC18 | Bacillus licheniformis | dephosphorylated linear DNA fragment + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Bacillus licheniformis |
| ? | x * 60000, calculated from amino acid sequence | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
the enzyme retains more than 60% activity at 80?C after 1 h incubation | Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
4-nitrophenyl phosphate | at pH 10.0 and 50°C | Bacillus licheniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 12 | more than 60% activity between pH 8.0 and 12.0 | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 13 | the enzyme maintains more than 80% activity after 1 h incubation at pH 7.0-13.0 | Bacillus licheniformis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus licheniformis | calculated from amino acid sequence | - |
9.1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5495 | - |
4-nitrophenyl phosphate | at pH 10.0 and 50°C | Bacillus licheniformis | |
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