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Literature summary for 3.1.3.1 extracted from

  • Iqbal, J.
    An enzyme immobilized microassay in capillary electrophoresis for characterization and inhibition studies of alkaline phosphatases (2011), Anal. Biochem., 414, 226-231.
    View publication on PubMed

Application

Application Comment Organism
biotechnology a simple and fast dynamically coated capillary electrophoretic method is developed for the characterization and inhibition studies of alkaline phosphatases Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
arsenate irreversible inhibitor Bos taurus
phosphate competitive inhibitor Bos taurus
theophylline reversible non-competitive inhibitor Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
4-nitrophenyl phosphate pH 9.5, 37°C, Vmax: 0.96 nmol/min/mg enzyme, CIAP immobilized enzyme reactor (in excellent agreement with a Km value of calf ALP enzyme using a 96-well microplate reader) Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Bos taurus
calf intestinal alkaline phosphatase
-
Bos taurus
CIAP
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
assay at Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000261
-
arsenate pH 9.5, 37°C, standard spectrophotometric assays Bos taurus
0.00241
-
phosphate pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay Bos taurus
0.00311
-
phosphate pH 9.5, 37°C, standard spectrophotometric assays Bos taurus
0.0089
-
arsenate pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay Bos taurus
0.05
-
theophylline pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay Bos taurus
0.096
-
theophylline pH 9.5, 37°C, standard spectrophotometric assays Bos taurus