Application | Comment | Organism |
---|---|---|
biotechnology | a simple and fast dynamically coated capillary electrophoretic method is developed for the characterization and inhibition studies of alkaline phosphatases | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenate | irreversible inhibitor | Bos taurus | |
phosphate | competitive inhibitor | Bos taurus | |
theophylline | reversible non-competitive inhibitor | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
4-nitrophenyl phosphate | pH 9.5, 37°C, Vmax: 0.96 nmol/min/mg enzyme, CIAP immobilized enzyme reactor (in excellent agreement with a Km value of calf ALP enzyme using a 96-well microplate reader) | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Bos taurus | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Bos taurus |
calf intestinal alkaline phosphatase | - |
Bos taurus |
CIAP | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000261 | - |
arsenate | pH 9.5, 37°C, standard spectrophotometric assays | Bos taurus | |
0.00241 | - |
phosphate | pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay | Bos taurus | |
0.00311 | - |
phosphate | pH 9.5, 37°C, standard spectrophotometric assays | Bos taurus | |
0.0089 | - |
arsenate | pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay | Bos taurus | |
0.05 | - |
theophylline | pH 9.5, 37°C, ALP immobilized capillary electrophoresis, Ki value is close to the Ki values obtained by the standard spectrophotometric assay | Bos taurus | |
0.096 | - |
theophylline | pH 9.5, 37°C, standard spectrophotometric assays | Bos taurus |