Protein Variants | Comment | Organism |
---|---|---|
additional information | HaALP is identified in the target insect gut brush border membrane vesicles as a receptor of the Bacillus thuringiensis coded Cry1Ac toxin. Lectin-ligand immunoblot assay detects the presence of alpha-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction | Helicoverpa armigera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cry1Ac toxin | environment friendly insecticidal crystal proteins encoded by the spore-forming bacteria Bacillus thuringiensis. Competitive inhibitor; environment friendly insecticidal crystal proteins encoded by the spore-forming bacteria Bacillus thuringiensis. Competitive inhibitor | Helicoverpa armigera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.58 | - |
4-nitrophenyl phosphate | - |
Helicoverpa armigera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
2 * 68000, SDS-PAGE | Helicoverpa armigera |
138000 | - |
gel filtration | Helicoverpa armigera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicoverpa armigera | B4Z1D6 | - |
- |
Helicoverpa armigera | B4Z1D7 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration chromatography using a pre-equilibrated column containing Sephadex G-150 | Helicoverpa armigera |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Helicoverpa armigera | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 68000, SDS-PAGE | Helicoverpa armigera |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Helicoverpa armigera |
HaALP | - |
Helicoverpa armigera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Helicoverpa armigera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.14 | - |
- |
Helicoverpa armigera |
10.3 | - |
assay at | Helicoverpa armigera |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000316 | - |
Cry1Ac toxin | competitive inhibition | Helicoverpa armigera |