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Literature summary for 3.1.3.1 extracted from

  • Mark, S.S.; Stolper, S.I.; Baratti, C.; Park, J.Y.; Taku, M.A.; Santiago-Aviles, J.J.; Kricka, L.J.
    Bioconjugation of alkaline phosphatase to mechanically processed, aqueous suspendible electrospun polymer nanofibers for use in chemiluminescent detection assays (2008), Macromol. Biosci., 8, 484-498.
    View publication on PubMed
Search for more literature for 3.1.3.1

Protein Variants

Protein Variants Comment Organism
biotechnology aqueous suspendible polymer nanostructures are prepared by simple microtome processing of electrospun nylon 6 nanofibers and are used to immobilize calf intestinal ALP by either covalent or noncovalent bioconjugation chemistries. Noncovalent immobilization of ALP to the mechanically cut nanofibers using a multi-stacked, layer-by-layer approach with the cationic polymer Sapphire II results in the highest enzyme loading. In terms of the overall catalytic performance of the various immobilized ALP systems, a single-stacked layer-by-layer assembly approach resulted in the highest level of enzymatic activity (30.1%) per unit mass of nanofiber support Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate layer-by-layer single-stack, immobilized ALP Bos taurus
0.04
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate direct physical adsorption, immobilized ALP Bos taurus
0.04
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate immobilized ALP Bos taurus
0.05
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate free ALP Bos taurus
0.1058
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate layer-by-layer multi-stack, immobilized ALP Bos taurus
0.114
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate glutaraldehyde X-link DAH, immobilized ALP Bos taurus
0.1159
-
 3-(4-methoxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]-decan]-4-yl)phenyl phosphate glutaraldehyde X-link, immobilized ALP Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P19111
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
disodium 3-(4-meth- oxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]- decan]-4-yl)phenyl phosphate + H2O
-
 Bos taurus ?
-
 ?

Synonyms

Synonyms Comment Organism
ALP
-
 Bos taurus
intestinal alkaline phosphatase
-
 Bos taurus