Application | Comment | Organism |
---|---|---|
molecular biology | influence of membrane lipid environment on the activity of GPI-anchored enzymes is investigated with PLAP reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts. The highest Vmax is recorded for a phosphatidylcholine/10% monosialoganglioside, while the lowest for a phosphatidylcholine/30% cholesterol mixture and for raft-mimicking 1:1:1 phosphatidylcholine/sphingolipid/cholesterol liposomes. No significant differences in Km are detected. The GPI-enzyme activity is affected by membrane curvature | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified from human placental tissue extract. The purity of PLAP after a gel filtration step is above 97% | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
placental alkaline phosphatase | - |
Homo sapiens |
PLAP | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Homo sapiens |