Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Sesana, S.; Re, F.; Bulbarelli, A.; Salerno, D.; Cazzaniga, E.; Masserini, M.
    Membrane features and activity of GPI-anchored enzymes: alkaline phosphatase reconstituted in model membranes (2008), Biochemistry, 47, 5433-5440.
    View publication on PubMed

Application

Application Comment Organism
molecular biology influence of membrane lipid environment on the activity of GPI-anchored enzymes is investigated with PLAP reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts. The highest Vmax is recorded for a phosphatidylcholine/10% monosialoganglioside, while the lowest for a phosphatidylcholine/30% cholesterol mixture and for raft-mimicking 1:1:1 phosphatidylcholine/sphingolipid/cholesterol liposomes. No significant differences in Km are detected. The GPI-enzyme activity is affected by membrane curvature Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from human placental tissue extract. The purity of PLAP after a gel filtration step is above 97% Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
placental alkaline phosphatase
-
Homo sapiens
PLAP
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
assay at Homo sapiens