Cloned (Comment) | Organism |
---|---|
Escherichia coli alkaline phosphatase is fused to a C-terminal region of Pseudomonas sp. MIS38 lipase (PML) and examined for secretion using the Escherichia coli cells carrying the heterologous type I secretion system. PML is one of the passenger proteins of TISS and contains 12 repetitive sequences and a secretion signal at the C-terminal region. The fusion protein is efficiently secreted to the extracellular medium, while alkaline phosphatase is not secreted at all, indicating that the secretion of alkaline phosphatase is promoted by a secretion signal of Pseudomonas sp. MIS38 lipase. The fusion protein purified from the culture supernatant existed as a homodimer, like AP, and is indistinguishable from alkaline phosphatase in enzymatic properties and stability | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | wild-type enzyme and alkaline phosphatase fused to a C-terminal region of Pseudomonas sp. MIS38 lipase | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 85 | 30°C: about 60% of maximal activity, 85°C: about 40% of maximal activity, wild-type enzyme and alkaline phosphatase fused to a C-terminal region of Pseudomonas sp. MIS38 lipase | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
10 min, pH 8.5, containing 10 mM MgCl2 and 1 mM ZnSO4, about 50% loss of activity | Escherichia coli |
95 | - |
10 min, pH 8.5, containing 10 mM MgCl2 and 1 mM ZnSO4, aout 80% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
wild-type enzyme and alkaline phosphatase fused to a C-terminal region of Pseudomonas sp. MIS38 lipase | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 60% of maximal activity, pH 9.5: about 65% of maximal activity, wild-type enzyme and alkaline phosphatase fused to a C-terminal region of Pseudomonas sp. MIS38 lipase | Escherichia coli |