Application | Comment | Organism |
---|---|---|
food industry | quantification of alkaline phosphatase by using a monoclonal antibody-based immunoassay immunoassay is appropriate for determining mild time/temperature treatment of milk and for the control of milk pasteurization | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Bos taurus | - |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, aout 13% loss of activity | Bos taurus |
56 | - |
a monoclonal antibody based capture immunoassay is used to study the kinetics of alkaline phosphatase heat denaturation in bovine milk over a range 50-60°C for 5 to 60 min using a colorimetric quantification of the enzyme activity as a reference test. A denaturation midpoint is obtained at 56°8C for a 30 min heating | Bos taurus |
60 | - |
30 min, 96.5% loss of activity | Bos taurus |