Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
T81A | the T81A mutant displays a somewhat lower affinity than the wild-type enzyme for both substrate and phosphate, while kcat does not change significantly | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
4-nitrophenyl phosphate | 25°C, pH 8, mutant enzyme T81A | Escherichia coli | |
0.0078 | - |
4-nitrophenyl phosphate | 25°C, pH 8, wild-type enzyme | Escherichia coli | |
0.16 | - |
4-nitrophenyl phosphate | 25°C, pH 8, wild-type enzyme | Escherichia coli | |
0.23 | - |
4-nitrophenyl phosphate | 25°C, pH 8, mutant enzyme T81A | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
4-nitrophenyl phosphate | 25°C, pH 8, mutant enzyme T81A | Escherichia coli | |
18 | - |
4-nitrophenyl phosphate | 25°C, pH 10.5, mutant enzyme T81A | Escherichia coli | |
24.39 | - |
4-nitrophenyl phosphate | 25°C, pH 10.5, mutant enzyme T81A | Escherichia coli | |
26 | - |
4-nitrophenyl phosphate | 25°C, pH10.5, wild-type enzyme | Escherichia coli | |
34 | - |
4-nitrophenyl phosphate | 25°C, pH 8, wild-type enzyme | Escherichia coli | |
36.98 | - |
4-nitrophenyl phosphate | 25°C, pH 8, mutant enzyme T81A | Escherichia coli | |
61.84 | - |
4-nitrophenyl phosphate | 25°C, pH 8, wild-type enzyme | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
phosphate | 25°C, pH 8, wild-type enzyme | Escherichia coli | |
0.0065 | - |
phosphate | 25°C, pH 8, mutant enzyme T81A | Escherichia coli | |
0.1 | - |
phosphate | 25°C, pH 10.5, wild-type enzyme | Escherichia coli | |
0.14 | - |
phosphate | 25°C, pH 10.5, mutant enzyme T81A | Escherichia coli |