Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3), full-length PhoX, and a truncated form that lacks the signal peptide | Pasteurella multocida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.01 mM, complete inhibition. Ca2+ is the best metal ion to reconstitute enzyme activity, followed by Co2+, Ni2+, Mn2+, and Mg2+. Zn2+, in contrast, failed to restore enzyme activity | Pasteurella multocida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates. D527 of PhoX might be the ligand bound to the catalytic calcium | Pasteurella multocida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Pasteurella multocida |
72800 | - |
2 * 72800, SDS-PAGE | Pasteurella multocida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pasteurella multocida | A1C3J6 | - |
- |
Pasteurella multocida X-73 | A1C3J6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pasteurella multocida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.78 | - |
- |
Pasteurella multocida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + H2O | - |
Pasteurella multocida | adenosine + phosphate | - |
? | |
AMP + H2O | - |
Pasteurella multocida X-73 | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Pasteurella multocida | ? | - |
? | |
ATP + H2O | - |
Pasteurella multocida X-73 | ? | - |
? | |
beta-glycerophosphate + H2O | - |
Pasteurella multocida | glycerol + phosphate | - |
? | |
beta-glycerophosphate + H2O | - |
Pasteurella multocida X-73 | glycerol + phosphate | - |
? | |
bis-p-nitrophenyl phosphate + H2O | - |
Pasteurella multocida | ? | - |
? | |
bis-p-nitrophenyl phosphate + H2O | - |
Pasteurella multocida X-73 | ? | - |
? | |
D-glucose 1-phosphate + H2O | - |
Pasteurella multocida | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Pasteurella multocida | D-glucose + phosphate | - |
? | |
diphosphate + H2O | - |
Pasteurella multocida | 2 phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Pasteurella multocida | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Pasteurella multocida X-73 | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 2 * 72800, SDS-PAGE | Pasteurella multocida |
Synonyms | Comment | Organism |
---|---|---|
phox | - |
Pasteurella multocida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pasteurella multocida |