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Literature summary for 3.1.3.1 extracted from

  • Wu, J.R.; Shien, J.H.; Shieh, H.K.; Hu, C.C.; Gong, S.R.; Chen, L.Y.; Chang, P.C.
    Cloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (PhoX) from Pasteurella multocida strain X-73 (2007), FEMS Microbiol. Lett., 267, 113-120.
    View publication on PubMed
Search for more literature for 3.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3), full-length PhoX, and a truncated form that lacks the signal peptide Pasteurella multocida

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.01 mM, complete inhibition. Ca2+ is the best metal ion to reconstitute enzyme activity, followed by Co2+, Ni2+, Mn2+, and Mg2+. Zn2+, in contrast, failed to restore enzyme activity Pasteurella multocida

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates. D527 of PhoX might be the ligand bound to the catalytic calcium Pasteurella multocida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Pasteurella multocida
72800
-
 2 * 72800, SDS-PAGE Pasteurella multocida

Organism

Organism UniProt Comment Textmining
Pasteurella multocida A1C3J6
-
-
Pasteurella multocida X-73 A1C3J6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pasteurella multocida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.78
-
-
 Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O
-
 Pasteurella multocida adenosine + phosphate
-
 ?
AMP + H2O
-
 Pasteurella multocida X-73 adenosine + phosphate
-
 ?
ATP + H2O
-
 Pasteurella multocida ?
-
 ?
ATP + H2O
-
 Pasteurella multocida X-73 ?
-
 ?
beta-glycerophosphate + H2O
-
 Pasteurella multocida glycerol + phosphate
-
 ?
beta-glycerophosphate + H2O
-
 Pasteurella multocida X-73 glycerol + phosphate
-
 ?
bis-p-nitrophenyl phosphate + H2O
-
 Pasteurella multocida ?
-
 ?
bis-p-nitrophenyl phosphate + H2O
-
 Pasteurella multocida X-73 ?
-
 ?
D-glucose 1-phosphate + H2O
-
 Pasteurella multocida D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Pasteurella multocida D-glucose + phosphate
-
 ?
diphosphate + H2O
-
 Pasteurella multocida 2 phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pasteurella multocida p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pasteurella multocida X-73 p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 2 * 72800, SDS-PAGE Pasteurella multocida

Synonyms

Synonyms Comment Organism
phox
-
 Pasteurella multocida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Pasteurella multocida