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Literature summary for 3.1.3.1 extracted from

  • Fadiloglu, S.; Erkmen, O.; Sekeroglu, G.
    Thermal and carbon dioxide inactivation of alkaline phosphatase in buffer and milk (2004), Food Technol. Biotechnol., 42, 27-32.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CO2 enzyme in buffer can be inactivated by COS treatment at atmospheric pressure, increasing inactivation, when temperature increases from 20°C to 50°C, 16% loss of activity after 30 min at 20°C, 81% loss of activity after 30 min at 50°C. Change in activity dependent on CO2 treatment is not observed in raw milk mainly due to strong buffering capacity of milk Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
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-
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Source Tissue

Source Tissue Comment Organism Textmining
intestinal mucosa
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Bos taurus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
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?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
88% loss of activity after 55 min in glycine buffer, 72% loss of activity after 120 min in pasteurized milk Bos taurus
60
-
complete inactivation after 50 min in glycine buffer, 15% loss of activity after 120 min in pasteurized milk Bos taurus
70
-
complete inactivation after 20 min in glycine buffer, complete inactivation after 40 min in pasteurized milk Bos taurus
80
-
complete inactivation after 4 min in glycine buffer, complete inactivation after 10 min in pasteurized milk Bos taurus