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Literature summary for 3.1.3.1 extracted from
- Purification and characterization of an esterase isozyme involved in hydrolysis of organophosphorus compounds from an insecticide resistant pest, Helicoverpa armigera (Lepidoptera: Noctueidae) (2006), Biochim. Biophys. Acta, 1760, 310-317.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | 1 mM, complete inactivation | Helicoverpa armigera |  |
| Ca2+ | 1 mM, 50% inhibition | Helicoverpa armigera | |
| Cd2+ | 1 mM, complete inactivation | Helicoverpa armigera | |
| Co2+ | 1 mM, 54% inhibition | Helicoverpa armigera | |
| Cu2+ | 1 mM, 70% inhibition | Helicoverpa armigera | |
| EDTA | 1 mM, complete inactivation | Helicoverpa armigera | |
| Hg2+ | 1 mM, complete inactivation | Helicoverpa armigera | |
| L-Phe | 1 mM, 13% inhibition | Helicoverpa armigera | |
| Mg2+ | 1 mM, 19% inhibition | Helicoverpa armigera | |
| Mn2+ | 1 mM, 43% inhibition | Helicoverpa armigera | |
| Mo2+ | 1 mM, 50% inhibition | Helicoverpa armigera | |
| sodium deoxycholate | 1 mM, 20% inhibition | Helicoverpa armigera | |
| VO3- | 1 mM, 78% inhibition | Helicoverpa armigera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.238 | - |
4-nitrophenyl phosphate | pH 7.4, 37°C | Helicoverpa armigera | |
| 0.348 | - |
paraoxon | pH 7.4, 37°C | Helicoverpa armigera | |
| 1.9 | - |
beta-Glycerophosphate | pH 7.4, 37°C | Helicoverpa armigera | |
| 2.26 | - |
ADP | pH 7.4, 37°C | Helicoverpa armigera | |
| 2.38 | - |
ATP | pH 7.4, 37°C | Helicoverpa armigera | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 1 mM, 13% activation | Helicoverpa armigera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Helicoverpa armigera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicoverpa armigera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Helicoverpa armigera |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33 | - |
- |
Helicoverpa armigera |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several weeks | Helicoverpa armigera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Helicoverpa armigera | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | - |
Helicoverpa armigera | ? | - |
? | |
| ATP + H2O | - |
Helicoverpa armigera | ? | - |
? | |
| beta-glycerophosphate + H2O | - |
Helicoverpa armigera | ? | - |
? | |
| paraoxon + H2O | - |
Helicoverpa armigera | diethylphosphate + 4-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Helicoverpa armigera |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
unstable above | Helicoverpa armigera |
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