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Literature summary for 3.1.3.1 extracted from
- Purification and enzymatic characterization of alkaline phosphatase from Pinctada fucata (2002), J. Mol. Catal. B, 17, 65-74.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Pinctada fucata |  |
| Cu2+ | - |
Pinctada fucata | |
| phosphate | competitive inhibition | Pinctada fucata | |
| WO4 3- | competitive inhibition, product analogue | Pinctada fucata | |
| Zn2+ | - |
Pinctada fucata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Pinctada fucata | |
| 2.86 | - |
4-nitrophenyl phosphate | pH 9.7, 37°C | Pinctada fucata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Pinctada fucata | |
| Co2+ | - |
Pinctada fucata | |
| Mg2+ | - |
Pinctada fucata | |
| Mn2+ | - |
Pinctada fucata | |
| additional information | not affected by monovalent cations | Pinctada fucata |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Pinctada fucata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pinctada fucata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 101fold, to homogeneity | Pinctada fucata |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Pinctada fucata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | purified enzyme | Pinctada fucata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Pinctada fucata | 4-nitrophenol + phosphate | - |
? | |
| additional information | the enzyme is a nonspecific phosphomonoesterase that functions through a phosphoseryl intermediate to produce free inorganic phosphate or to transfer the phosphoryl group to other alcohols | Pinctada fucata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Pinctada fucata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphatase | - |
Pinctada fucata |
| ALP | - |
Pinctada fucata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pinctada fucata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.7 | - |
- |
Pinctada fucata |
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