Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Morales, A.C.; Nozawa, S.R.; Thedei, G.Jr.; Maccheroni, W.Jr.; Rossi, A.
    Properties of a constitutive alkaline phosphatase from strain 74A of the mold Neurospora crassa (2000), Braz. J. Med. Biol. Res., 33, 905-912.
    View publication on PubMed
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
 Neurospora crassa
histidine
-
 Neurospora crassa
Tris-HCl buffer
-
 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 4-nitrophenyl phosphate pH 8.9, 37°C Neurospora crassa
0.44
-
 Alpha-naphthyl phosphate pH 8.9, 37°C Neurospora crassa
0.47
-
 L-histidinyl phosphate pH 8.9, 37°C Neurospora crassa
2.46
-
 beta-glyceryl phosphate pH 8.9, 37°C Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Be2+
-
 Neurospora crassa
Mg2+ activation Neurospora crassa
Zn2+ activation Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 1 * 56000, SDS-PAGE Neurospora crassa
58000
-
 gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
 constitutive enzyme expression
-
Neurospora crassa St. L. 74A
-
 constitutive enzyme expression
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 131.4fold Neurospora crassa

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Neurospora crassa
a

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell most efficient in presence of saturating phosphate at pH 7.1 Neurospora crassa
-
mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
223.3
-
 purified enzyme Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Neurospora crassa 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Neurospora crassa St. L. 74A 4-nitrophenol + phosphate
-
 ?
alpha-naphthyl phosphate + H2O
-
 Neurospora crassa alpha-naphthol + phosphate
-
 ?
alpha-naphthyl phosphate + H2O
-
 Neurospora crassa St. L. 74A alpha-naphthol + phosphate
-
 ?
beta-glyceryl phosphate + H2O
-
 Neurospora crassa glycerol + phosphate
-
 ?
beta-glyceryl phosphate + H2O
-
 Neurospora crassa St. L. 74A glycerol + phosphate
-
 ?
L-histidinyl phosphate + H2O
-
 Neurospora crassa L-histidinol + phosphate
-
 ?
L-histidinyl phosphate + H2O
-
 Neurospora crassa St. L. 74A L-histidinol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE Neurospora crassa

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Tris-HCl partly protects against heat inactivation Neurospora crassa
70
-
 half-life 19 min in Tris-HCl, 2.3 min in CHES Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10.5
-
 Neurospora crassa