Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2fold activation at 1 mM | Pyrococcus abyssi | |
Mg2+ | 2fold activation at 1 mM | Pyrococcus abyssi | |
Zn2+ | 2fold activation at 1 mM | Pyrococcus abyssi |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli HMS174 (DE3) | Pyrococcus abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 68% inhibition at 1 mM, 86% inhibition at 10 mM | Pyrococcus abyssi | |
Ca2+ | 17% inhibition at 1 mM | Pyrococcus abyssi | |
Cu2+ | 28% inhibition at 1 mM | Pyrococcus abyssi | |
DTT | nearly complete inhibition at 2 mM | Pyrococcus abyssi | |
EDTA | 90% inhibition at 1 mM | Pyrococcus abyssi | |
EGTA | 82% inhibition at 1 mM | Pyrococcus abyssi | |
KCl | 90% remaining activity | Pyrococcus abyssi | |
molybdate | slight inhibition at 10 mM | Pyrococcus abyssi | |
additional information | no inhibition by molybdate and vanadate | Pyrococcus abyssi | |
NaCl | 2 M, 60% remaining activity | Pyrococcus abyssi | |
Ni2+ | 56% inhibition at 1 mM | Pyrococcus abyssi | |
phosphate | - |
Pyrococcus abyssi | |
vanadate | - |
Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | highly dependent on divalent cations | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 54000, SDS-PAGE | Pyrococcus abyssi |
54000 | - |
2 * 54000, SDS-PAGE | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9UZV2 | euryarchaeon, strain Orsay, highly thermostable enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, 450fold, to 95% purity | Pyrococcus abyssi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Pyrococcus abyssi | 4-nitrophenol + phosphate | - |
? | |
additional information | the enzyme also dephosphorylizes cohesive and blunt ends of DNA fragments, cohesive ends are slightly preferred | Pyrococcus abyssi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Pyrococcus abyssi |
monomer | 1 * 54000, SDS-PAGE | Pyrococcus abyssi |
More | both monomeric and dimeric enzyme forms exist, no cysteine residues are involved in intramolecular disulfide bonds | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Pyrococcus abyssi |
AP | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pyrococcus abyssi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | 25% of maximal activity at 37°C, 80% of maximal activity at 60-80°C | Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
80% remaining activity after 36 h | Pyrococcus abyssi |
100 | - |
half-life 18 h | Pyrococcus abyssi |
105 | - |
half-life 5 h | Pyrococcus abyssi |
115 | - |
inactivation within 30 min | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Pyrococcus abyssi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 12 | - |
Pyrococcus abyssi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus abyssi | calculation from amino acid sequence | - |
4.9 |