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Literature summary for 3.1.3.1 extracted from
- Partial purification, characterisation and histochemical localisation of alkaline phosphatase from ascocarps of the edible desert truffle Terfezia claveryi Chatin (2009), Plant Biol., 11, 678-685.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| orthovanadate | 70% inhibition at 1 mM, competitive | Terfezia claveryi |  |
| Tartrate | 5.9% inhibition at 1 mM | Terfezia claveryi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
4-nitrophenyl phosphate | - |
Terfezia claveryi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Terfezia claveryi | - |
Chatin | - |
| Terfezia claveryi Chatin | - |
Chatin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fractional precipitation with polyethylene glycol (3-7% PEG 8000) | Terfezia claveryi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ascocarp | - |
Terfezia claveryi | - |
| gleba | peridium, hypothecium and ascogenic hyphae | Terfezia claveryi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
after partial purification | Terfezia claveryi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Terfezia claveryi | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Terfezia claveryi Chatin | 4-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphatase | - |
Terfezia claveryi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Terfezia claveryi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
1 h 45°C: 75% remaining activity | Terfezia claveryi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Terfezia claveryi |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0425 | - |
orthovanadate | - |
Terfezia claveryi | |
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