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Literature summary for 3.1.26.8 extracted from

  • Condon, C.; Brechemier-Baey, D.; Beltchev, B.; Grunberg-Manago, M.; Putzer, H.
    Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function (2001), RNA, 7, 242-253.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.8

Cloned(Commentary)

Cloned (Comment) Organism
gene yabF, overexpression in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information bacteria lacking the gene rnmV do not produce mature 5S RNA, but it is nonessential for cell viability and not necessary for ribosomal function Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5S precursor rRNA + H2O Bacillus subtilis enzyme is responsible for maturation of 5S rRNA mature 5S rRNA
-
 ?
additional information Bacillus subtilis the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 gene yabF, proposition to rename the gene rnmV
-

Purification (Commentary)

Purification (Comment) Organism
native from strain SSB306 containing a modified L6 protein, recombinant from Escherichia coli Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor reaction scheme Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5S precursor rRNA + H2O enzyme is responsible for maturation of 5S rRNA Bacillus subtilis mature 5S rRNA
-
 ?
5S precursor rRNA + H2O in contrast to the Escherichia coli enzyme, the Bacillus subtilis enzyme cleaves the 5S rRNA in a double-stranded region that extends from the L5 arm in one step Bacillus subtilis mature 5S rRNA
-
 ?
additional information the enzyme is highly conserved among G + C Gram-positive bacteria, phylogenetic distribution Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
5S ribosomal maturation nuclease
-
 Bacillus subtilis
5S ribosomal RNA maturase
-
 Bacillus subtilis
5S ribosomal RNA maturation endonuclease
-
 Bacillus subtilis
RNase M5
-
 Bacillus subtilis