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Literature summary for 3.1.26.8 extracted from

  • Meyhack, B.; Pace, B.; Uhlenbeck, O.C.; Pace, N.R.
    Use of T4 RNA ligase to construct model substrates for a ribosomal RNA maturation endonuclease (1978), Proc. Natl. Acad. Sci. USA, 75, 3045-3049.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5S rRNA precursor + H2O symmetrically arranged sequences are not used in the RNase M5 interaction Bacillus subtilis mature 5S rRNA + oligonucleotides
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5S rRNA precursor + H2O the enzyme cleaves twice in double-helical region of a 179-nucleotide precursor of 5S rRNA to yield mature 5S rRNA of 116 nucleotides plus fragments of 21 and 42 nucleotides. The conformation, not sequence, is the important feature in the locale of the cleaved bonds Bacillus subtilis mature 5S rRNA + oligonucleotides
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