Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a mutant enzymatically active enzyme P4 RNA ribozyme with altered metal ion specificity, preference for Ca2+ instead of Mg2+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics of RNA binding by wild-type and mutant ribozymes | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | preferred by mutant ribozyme, binding structure of the enzyme RNA P4 element, affinities of wild-type and mutant P4 element | Escherichia coli | |
Mg2+ | preferred by wild-type ribozyme, binding structure of the enzyme RNA P4 element, affinities of wild-type and mutant P4 element | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
functionally active enzyme RNA P4 element ribozyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | from Escherichia coli and Methylophilus methylotrophus | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural model of functionally active enzyme RNA P4 element ribozyme, metal ions binding structure, hairpin structure complexed with Mg2+, Ca2+, or cobalt(III)-hexamine, wild-type and mutant ribozymes, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
P4 | - |
Escherichia coli |
RNase P | - |
Escherichia coli |
RNase P RNA | - |
Escherichia coli |