Literature summary for 3.1.26.6 extracted from

Muller, W.E.G.
Endoribonuclease IV. A poly(A)-specific ribonuclease from chick oviduct. 1. Purification of the enzyme (1976), Eur. J. Biochem., 70, 241-248.

Search for more literature for 3.1.26.6

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	presence of 2 mM EDTA results in total inhibition, under 0.2 mM: no effect	Gallus gallus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	optimum: 1 mM	Gallus gallus
Mn2+	optimum: 0.5 mM, more effective than Mg2+	Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	gel filtration	Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
RNA + H2O	Gallus gallus	processing of nuclear RNA to mRNA	oligonucleotides	average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups	?

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
15000fold, nearly to homogeneity	Gallus gallus

Reaction

Reaction	Comment	Organism	Reaction ID
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups	forms oligonucleotides with an average chain length of 10	Gallus gallus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
oviduct	-	Gallus gallus	-

Storage Stability

Storage Stability	Organism
-20°C, in solution 5, addition of 1 mg/ml albumin, after 6 months 30% of activity were lost	Gallus gallus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	not: poly(C), poly(U), poly(G), double-stranded poly(A)*poly(U), DNA	Gallus gallus	?	-	?
poly(A) + H2O	-	Gallus gallus	oligonucleotides	with an average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups	?
RNA + H2O	processing of nuclear RNA to mRNA	Gallus gallus	oligonucleotides	average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Gallus gallus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	-	Gallus gallus

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	-	35% of optimum	Gallus gallus
9.5	-	40% of optimum	Gallus gallus

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Release 2023.1 (January 2023)